Effects of p27kip1 gene on DNA and protein synthesis in esophageal carcinoma cells |
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| Authors: | TONG Qiang WANG Xiao-hu XIE Guo-jian WU Qing-ming |
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| Institution: | Digestion Department, Taihe Hospital of Yunyang Medical College, Shiyan 442000, China |
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| Abstract: | AIM: To investigate the effects of p27kip1 gene on DNA replication and protein synthesis in esophageal carcinoma cells. METHODS: Recombinant adenovirus Ad-p27kip1 was constructed and transfected into esophageal carcinoma cell EC-9706, and its effects on DNA replication, protein synthesis and the growth of esophageal carcinoma cells were explored by means of cell growth count, 3H]-TdR, 3H]-Leucine incorporation method and flow cytometry. RESULTS: The growth of esophageal carcinoma cells was inhibited obviously. The radioactive intensity of -TdR and -Leucine in Ad-p27kip1 group decreased significantly than that in control group after EC-9706 transfection (P<0.01), while the multiplication of esophageal carcinoma cell was inhibited obviously. CONCLUSION: Ad-p27kip1 inhibited the multiplication of the esophageal carcinoma cells, which may be related to its suppressive function for DNA replication and protein synthesis. |
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| Keywords: | Genes p27kip1 Esophageal neoplasms DNA replication |
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