| 野葛异黄酮糖苷的分离纯化及体外清除自由基活性的研究 |
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| 引用本文： | 杨华,韩坤,宛晓春,方从兵.野葛异黄酮糖苷的分离纯化及体外清除自由基活性的研究[J].安徽农业大学学报,2011,38(2):151-155. |
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| 作者姓名： | 杨华 韩坤 宛晓春 方从兵 |
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| 作者单位： | 安徽农业大学农业部茶及药用植物安全生产重点开放实验室,合肥,230036;安徽农业大学理学院,合肥,230036;安徽农业大学农业部茶及药用植物安全生产重点开放实验室,合肥,230036;安徽农业大学农业部茶及药用植物安全生产重点开放实验室,合肥,230036;安徽农业大学园艺学院,合肥,230036 |
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| 基金项目： | 国家自然科学基金，安徽农业大学校长青年基金 |
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| 摘 要： | 以野葛的根样为试材,依次通过总黄酮提取、有机溶剂分级分离和柱色谱法进行分离纯化,获得4种异黄酮糖苷组分,依据核磁共振谱和质谱分析结果分别鉴定为葛根素、大豆苷、芒柄花苷和染料木苷。采用1,1-二苯基苦基苯肼(DPPH·)的有机自由基体系和超氧阴离子(O2-·)的无机活性氧自由基体系,对上述4种异黄酮糖苷化合物的体外清除自由基能力和抗氧化活性进行测定。结果表明,4个异黄酮糖苷组分对DPPH·自由基和O2-·自由基均具有一定的清除活性,清除能力的大小与处理浓度均成正相关关系,对DPPH·自由基的清除效果由高到低依次为染料木苷、大豆苷、葛根素和芒柄花苷,抑制O2-·自由基能力由强到弱依次为葛根素、大豆苷、染料木苷和芒柄花苷。
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| 关 键 词： | 异黄酮糖苷 分离纯化 清除自由基 野葛 |
Free radical scavenging capacities of isoflavonoid glycosides isolated from Pueraria Radix |
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| YANG Hu,HAN Kun,WAN Xiao-chun and FANG Cong-bing.Free radical scavenging capacities of isoflavonoid glycosides isolated from Pueraria Radix[J].Journal of Anhui Agricultural University,2011,38(2):151-155. |
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| Authors: | YANG Hu HAN Kun WAN Xiao-chun and FANG Cong-bing |
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| Institution: | 1,3 (1.Key Laboratory of Tea & Medicinal Plants and Product Safety,Ministry of Agriculture,Anhui Agricultural University,Hefei 230036; 2.School of Science,Anhui Agricultural University,Hefei 230036;3.School of Horticulture,Anhui Agricultural University,Hefei 230036) |
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| Abstract: | Crude total isoflavonoids extracted from Pueraria Radix were partitioned by different organic solvents, then isolated and purified by several column chromatographic techniques and four isoflavonoid glycosides were obtained. On basis of the NMR and ESI-MS spectra, these four compounds can be identified as puerarin, daidzin, ononin, and genistin, respectively. Using an organic free radical system of 1, 1-diphenyl- 2-picrylhydrazyl (DPPH·) and an inorganic free radical system of superoxide anion free radical, the free radical scavenging capacities and antioxidant activities of these four isoflavonoid glycosides were examined in vitro. The results showed that all the four isoflavonoid glycosides were discovered to possess the scavenging capacities of the DPPH· free radical and superoxide anion radical. Meanwhile, there was a significantly positive correlation between the two free radical scavenging capacities and the treated concentrations of the four isoflavonoid glycosides. Genistin showed the highest DPPH· free radical scavenging capacity, followed by daidzin and puerarin, and ononin was the lowest among them. However, the superoxide anion free radical scavenging capacities of the four isoflavonoid glycosides were different from the former. Puerarin was discovered to be the strongest among them, followed by daidzin, genistin and ononin. |
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| Keywords: | isoflavonoid glycoside isolation and purification free radical scavenging capacity Pueraria lobata (Willd) Ohwi |
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