| 杜仲翅果桃叶珊瑚苷高效液相色谱分析方法的建立 |
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| 引用本文： | 崔丽,龚志华,刘仲华,张盛,傅冬和,肖文军.杜仲翅果桃叶珊瑚苷高效液相色谱分析方法的建立[J].湖南农业大学学报(自然科学版),2013,39(1):86-90. |
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| 作者姓名： | 崔丽 龚志华 刘仲华 张盛 傅冬和 肖文军 |
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| 作者单位： | 湖南农业大学植物资源工程系,湖南农业大学园艺园林学院,湖南农业大学国家植物功能成分工程技术研究中心,湖南农业大学园艺园林学院植物资源工程系,湖南农业大学园艺园林学院植物资源工程系,湖南农业大学园艺园林学院植物资源工程系 |
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| 摘 要： | 为建立检测杜仲翅果桃叶珊瑚苷含量的HPLC法，以杜仲翅果为原料，采用溶剂浸提法，用85%乙醇在提取温度50 ℃、料液比1∶20、提取时间30 min的条件下提取2次，浓缩提取液至无醇味，综合分析检测波长、流动相、流速、检测温度4个因素对HPLC图谱的影响。结果表明：在C18色谱柱(150 mm×4.6 mm，5 µm)、检测波长208 nm、柱温20 ℃、流速0.8 mL/min的条件下，用1%～10%的乙腈梯度洗脱30 min，杜仲翅果桃叶珊瑚苷含量在样品进样量0.30～1.50 µg与峰面积呈良好的线性关系，R2 =0.999 6，平均回收率为84.3%，相对标准差为1.97%(n=5)。该方法可准确检测杜仲翅果乙醇提取物中桃叶珊瑚苷含量，具有样品处理简单、稳定性强、重现性好、精密度高的优点。
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| 关 键 词： | 杜仲翅果 桃叶珊瑚苷 高效液相色谱 |
| 收稿时间： | 2012/6/25 0:00:00 |
| 修稿时间： | 2012/8/14 0:00:00 |
Detection of aucubin in samara of Eucommia ulmoides byhigh performance liquid chromatography |
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| XIAO Shu-ping,SUN Ya-hong,Zhang min.Detection of aucubin in samara of Eucommia ulmoides byhigh performance liquid chromatography[J].Journal of Hunan Agricultural University,2013,39(1):86-90. |
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| Authors: | XIAO Shu-ping SUN Ya-hong Zhang min |
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| Institution: | Botanic Resource Engineering Department of Hunan Agricultural University,,,,, |
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| Abstract: | Taking financial date of 45 listed in automobile manufacturing industry from 2010 to 2011 as samples, the research has been done. It is discovered that the turnover tax burden were 0.018,0.0075, and the income tax burden were 0.1951,0.2079, moreover, the income tax burden varies abnormally. The empirical results show that the main business income and return on equity have a positive influence on the turnover tax burden, while main business cost and the variation rate of inventory have a negative influence on the turnover tax burden, besides asset-liability ratio has negative impacts on income tax burden, but the size of the enterprise, the density of fixed assets and return on equity have a positive influence on the income tax. |
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| Keywords: | samara of Eucommia ulmoides aucubin high performance liquid chromatography(HPLC) |
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