| 水稻dirigent基因家族生物信息学分析 |
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| 引用本文： | 穰中文,周清明.水稻dirigent基因家族生物信息学分析[J].湖南农业大学学报(自然科学版),2013,39(2):111-120. |
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| 作者姓名： | 穰中文 周清明 |
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| 作者单位： | 湖南农业大学,湖南农业大学 |
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| 摘 要： | 摘 要：利用现有的水稻生物信息资源，共鉴定出了53个水稻dirigent (OsDIR)基因，它们分布在8条水稻染色体上；基因结构分析显示，有32个OsDIR基因不含内含子，占总数的60.4%；保守功能区域预测表明，OsDIR基因至少含有1个保守的DIR功能域；模块预测显示，水稻DIR蛋白拥有至少10个大小不同的保守模块，且不同模块在基因家族成员中出现的频率有较大的差异；蛋白序列比对表明，该基因家族蛋白保守序列均位于DIR功能域内；蛋白功能预测表明，大多数OsDIR蛋白为稳定的疏水性蛋白，表达于大多数细胞器中，且在细胞壁中表达最为丰富；同源基因分子遗传进化分析表明，OsDIR基因可分为5个亚类，功能域片段与基因的复制特征表明，OsDIR基因可能起源于共同的祖先(基因)。
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| 关 键 词： | 水稻 dirigent基因 基因家族 生物信息学 |
| 收稿时间： | 2011/12/21 0:00:00 |
| 修稿时间： | 2012/11/27 0:00:00 |
Bioinformatic analysis of the dirigent gene family in riceRANG Zhong-wen, ZHOU Qing-ming* |
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| Institution: | Hunan Agricultural University, |
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| Abstract: | Fifty-three OsDIR genes distributed on 8 different rice chromosomes were identified according to rice bioinformatics resource. Gene structural analyses showed that 32 OsDIRs, accounting for 60.4% of the total, are intron-less gene; conserved domain prediction demonstrated that each of the OsDIR genes contains at least one conserved dirigent domain and 10 conserved motifs with different sizes, among which each motif showed different frequencies in all members of OsDIR gene family, were predicted through motif analysis. Multiple alignment of protein sequences showed that all the conserved protein sequences were located in the conserved domain, and most of the proteins are stabilized hydrophobic proteins, which expressed in most of the organelles with the most of which abundantly expressed in the cell wall, according to OsDIR protein sequences functional prediction. The OsDIR genes were classified into five subgroups according to phylogenetic analyses based on conserved protein domain sequences. Additionally, the replication characteristics of domain fragments and genes indicated that the OsDIR genes origin from the common ancestor/gene. |
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| Keywords: | Key words: rice dirigent gene gene family bioinformatics |
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