| 马蹄纹天竺葵异倍型嵌合体不同层体细胞染色体数目的直接测定 |
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| 引用本文： | 李明银.马蹄纹天竺葵异倍型嵌合体不同层体细胞染色体数目的直接测定[J].林业科学,2007,43(7):125-127. |
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| 作者姓名： | 李明银 |
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| 作者单位： | 绵阳师范学院园林科学研究所,绵阳,621000;德国柏林洪堡大学园林科学研究所,柏林,14195 |
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| 基金项目： | Supported by Scientific Research from Mianyang Normal University (MA2005008) and from Sichuan Education administration(2006C-048) . |
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| 摘 要： | Shoot meristem of many flowering plant species consists of threeindependent cell layers (L1 ,L2 and L3) (Tilney-Bassett ,1986) .The cells inthetwo outerlayers (L1 ,L2) divide anticlinallyinthe samelayer whilethoseininnerlayer (L3) divideinany plane (Bartonet al.,1993 ;Li ,2005) .If the genotypes of cells at least twolayers in a plant meristemare different ,theplantis called chimera (Tilney-Bassett ,1986 ; Burgeet al.,2002) . When one or more entire cell layer(s) are geneticallydifferentfr…
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| 关 键 词： | 马蹄纹天竺葵 倍型嵌合体 组织切片 染色体倍数 细胞分裂 |
| 文章编号： | 1001-7488（2007）07-0125-03 |
| 修稿时间： | 2006-03-29 |
A Method for Direct Determination of Ploidy Level of Somatic Cells in Different Layers of Cytochimeral Tissue of Pelargonium zonale |
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| Li Mingyin.A Method for Direct Determination of Ploidy Level of Somatic Cells in Different Layers of Cytochimeral Tissue of Pelargonium zonale[J].Scientia Silvae Sinicae,2007,43(7):125-127. |
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| Authors: | Li Mingyin |
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| Institution: | 1.Institute of Garden Science, Mianyang Normal University Mianyang 621000; 2.Institute of Garden Science, Humboldt University of Berlin Berlin, Germany 14195 |
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| Abstract: | Cytochimeras are important materials used to study the behaviors of the cells with different ploidies in a tissue. The epidermal cell size and pollen size are commonly used to indirect detective the cell ploidy levels in L1 and L2 layers, because ploidy is one of the parameters that influence cell size; higher ploidy levels usually result in proportionally larger cells. To my knowledge, there is not any published report for direct determining ploidy of the somatic cells in different layers of a cytochimera.During a histological investigation of Pelargonium zonale 'little lady', a method was developed, which was able to directly determine ploidy by counting chromosome number of the somatic cells in different layers of cytochimeral tissue in embedding slices stained with Toluidin blue. In the mean time, the processes of nuclear division in cytochimeral tissue were observed with this method. Therefore, it can not only be used to directly determine the ploidy of the somatic cells in different layers in cytochimera, but also be used for study of cell division procedures of mutated cells in cytochimeras. |
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| Keywords: | Pelargonium zonale cytochimera tissue section ploidy level cell division |
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