| 利用秋水仙素喷淋加倍不同遗传基础的单倍体子粒探讨 |
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| 引用本文： | 孙成韬,孙楠,马云祥,姜敏,刘晓丽,王延波.利用秋水仙素喷淋加倍不同遗传基础的单倍体子粒探讨[J].玉米科学,2020,28(3):66-70. |
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| 作者姓名： | 孙成韬 孙楠 马云祥 姜敏 刘晓丽 王延波 |
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| 作者单位： | 辽宁省农业科学院玉米研究所, 沈阳 110161 |
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| 基金项目： | 国家重点研发计划项目“七大农作物育种”（2017YFD0101103，2017YFD0101103-1） |
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| 摘 要： | 以不同血缘及不同世代的遗传育种材料为母本、高频孤雌生殖诱导系辽诱1和CAU5等为父本杂交诱导产生的单倍体子粒为实验材料,研究秋水仙素浓度为0.625 mg/mL的条件下,用喷淋加倍设备对试验材料进行8 h喷淋的加倍效果。结果表明,喷淋秋水仙素后,郑单958的S2代的材料加倍率最高,为33.75%;第2位的是L3258×H71,是NSS种质S0代材料,其加倍率为13.02%;第3位的是D299/PH4CV,为NSS种质S1代材料,加倍率为10.34%。研究表明,喷淋秋水仙素的加倍效果与遗传背景关系紧密,喷淋秋水仙素加倍率总体由高到低的顺序为郑单958NSS种质(SS+NSS)种质SS种质欧洲杂交种,喷淋秋水仙素对全部试验材料的加倍率都有提高作用,比自然加倍提高0.83～5.08倍。利用该喷淋设备加倍单倍体子粒,具有节约用药量、操作简便、便于大规模生产应用、减少人员对秋水仙素的接触、废液回收方便、有利于保护环境等优点。
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| 关 键 词： | 玉米 单倍体 种质基础 秋水仙素 加倍率 |
| 收稿时间： | 2019/6/21 0:00:00 |
Exploration of Haploid Seeds with Different Genetic Maize Breeding Materials by Spraying Colchicine |
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| SUN Cheng-tao,SUN Nan,MA Yun-xiang,JIANG Min,LIU Xiao-li,WANG Yan-bo.Exploration of Haploid Seeds with Different Genetic Maize Breeding Materials by Spraying Colchicine[J].Journal of Maize Sciences,2020,28(3):66-70. |
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| Authors: | SUN Cheng-tao SUN Nan MA Yun-xiang JIANG Min LIU Xiao-li WANG Yan-bo |
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| Institution: | Institute of Maize Research, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China |
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| Abstract: | Using different generations of genetic breeding materials with different genetic background as female parent and high-frequency parthenogenetic induction line G5 as haploid induced by male parent hybridization as experimental materials, the spraying doubling equipment was used to spray the experimental materials for 8 hours under the condition of colchicine concentration of 0.625 mg/mL. After spraying colchicine, the results showed that the material doubling rate of Zhengdan958 in S2 generation was the highest, at 33.75%. Second place was L3258×H71 which was added to NSS germplasm such as Ludahonggu and then to yellow-modified S0 generation. The doubling rate was 13.02%. The third place was that NSS germplasm added to tropical germplasm S1 generation. The doubling rate was 10.34%. The results showed that the doubling effect of spraying colchicine was closely related to the genetic background. In this study, the overall order of the doubling rate of spraying colchicine was Zhengdan958 > NSS germplasm > (SS+NSS) germplasm > SS germplasm > European hybrid. Spraying colchicine could improve the doubling rate of all the experimental materials, which was higher than natural doubling 0.83-5.08 times. Using the spray equipment to double the haploid seeds has the advantages of saving the dosage, simple operation, convenient for large-scale production and application, reducing the contact of personnel with colchicine, convenient waste liquid recovery, and environmental protection. |
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| Keywords: | Maize Haploid Germplasm base Colchicine Doubling rat |
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