| 猪精子冷冻损伤与保护剂的研究进展 |
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| 引用本文： | 张群,王骞,郭铭晖,王硕,魏华凯,李雁冰,黄大鹏,魏国生,李井春.猪精子冷冻损伤与保护剂的研究进展[J].猪业科学,2021,38(2):106-108. |
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| 作者姓名： | 张群 王骞 郭铭晖 王硕 魏华凯 李雁冰 黄大鹏 魏国生 李井春 |
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| 作者单位： | 黑龙江八一农垦大学动物科技学院,黑龙江 大庆 163319;黑龙江八一农垦大学动物科技学院,黑龙江 大庆 163319;黑龙江八一农垦大学动物科技学院,黑龙江 大庆 163319;黑龙江八一农垦大学动物科技学院,黑龙江 大庆 163319;黑龙江八一农垦大学动物科技学院,黑龙江 大庆 163319;黑龙江八一农垦大学动物科技学院,黑龙江 大庆 163319;黑龙江八一农垦大学动物科技学院,黑龙江 大庆 163319;黑龙江八一农垦大学动物科技学院,黑龙江 大庆 163319;黑龙江八一农垦大学动物科技学院,黑龙江 大庆 163319 |
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| 基金项目： | 黑龙江省博士后启动基金（LBH-Q18100）；黑龙江八一农垦大学博士启动基金（XYB-2016-04）；黑龙江省大学生创新创业训练计划项目（C类国家级，项目编号：202010223044） |
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| 摘 要： | 猪精液冷冻保存技术主要是利用液氮(-196℃)作为冷源,在超低温的环境下使精子生理代谢完全暂停,实现长期保存的目的。猪精子在低温环境主要损伤是细胞内结冰晶造成的机械损伤、游离的活性氧造成的化学损伤,对这两种损伤机制研究是国内外科研工作者们的研究热点。文章主要简述猪精液冷冻技术发展简况,精子在冷冻过程中受损伤机制和现阶段应用几种保护剂。
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| 关 键 词： | 精液 冷冻保存 氧化损伤 影响因素 |
| 收稿时间： | 2020/12/27 0:00:00 |
Research progress on cryogenic damage and protective agent of porcine sperm |
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| Qun Zhang,Qian Wang,Minghui Guo,Shuo Wang,Huakai Wei,Yanbing Li,Dapeng Huang,Guosheng Wei and Jingchun Li.Research progress on cryogenic damage and protective agent of porcine sperm[J].Swine Industry Science,2021,38(2):106-108. |
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| Authors: | Qun Zhang Qian Wang Minghui Guo Shuo Wang Huakai Wei Yanbing Li Dapeng Huang Guosheng Wei and Jingchun Li |
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| Institution: | College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China,College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China,College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China,College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China,College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China,College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China,College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China,College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China and College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China |
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| Abstract: | Cryopreservation technology of porcine sperm mainly uses liquid nitrogen(-196℃)as the cold source to completely suspend the physiological metabolism of sperm in the ultra-low temperature environment,so as to achieve the purpose of long-term preservation.In low temperature environment,porcine spermatozoa is mainly damaged by ice crystals inside cells and by free reactive oxygen species.This paper mainly describes the development of pig semen Cryopreservation technology,the mechanism of sperm damage during freezing and the application of several protective agents at present. |
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| Keywords: | Semen Cryopreservation Oxidative damage Infl uencing factors |
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