| 花生种子相对分子质量17500多肽抗体制备及其应用 |
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| 引用本文： | 芦春斌,乔爱民.花生种子相对分子质量17500多肽抗体制备及其应用[J].仲恺农业技术学院学报,2000,13(3):5-9. |
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| 作者姓名： | 芦春斌 乔爱民 |
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| 作者单位： | [1]暨南大学生殖免疫中心 [2]仲恺农业技术学院园艺系 |
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| 基金项目： | 广东省自然科学基金资助项目!(95 0 0 90 ) |
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| 摘 要： | 用一种纯化的花生种子高甲硫氨酸蛋白－相对分子质量１７５００多肽为抗原，免疫动物获得了高特异性的抗血清，以之为探针分析比较了其它几种豆科植物中们花生球蛋白Ⅱ提取物，免疫亲合反应均呈阳性，而玉米种子呈阴性反应，表明该多肽是豆科植物种子蛋白保守成分，Ｗｅｓｔｅｒｎ ｂｌｏｔ分析表明该多肽在高甲硫氨酸类型代表品种和低甲硫氨酸类型代表品种中均存在。
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| 关 键 词： | 花生种子 多肽 免疫亲合反应 |
Preparation of Mr 17 500 polypeptide antibody and its application |
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| LU Chun bin ,QIAO Ai min ,HUANG Shang zhi ,FU Jia rui.Preparation of Mr 17 500 polypeptide antibody and its application[J].Journal of Zhongkai Agrotechnical College,2000,13(3):5-9. |
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| Authors: | LU Chun bin QIAO Ai min HUANG Shang zhi FU Jia rui |
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| Institution: | LU Chun bin 1,QIAO Ai min 2,HUANG Shang zhi 3,FU Jia rui 3 |
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| Abstract: | Antibody from peanut seeds was raised in rabbit. ELISA using the antibody against peanut Mr 17 500 polypeptide for conarachin II in seeds of soybean, mung bean, cowpea and corn was analyzed. Results showed that there were immunological cross reactions of that antibody with the protein components in the soybean, mung bean and cowpea seeds, but no immunological cross reaction with the protein components in corn. Western blot showed that there was Mr 17 500 polypeptide in both peanut cultivars with different methioine composition. |
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| Keywords: | peanut seed polypeptide immunological cross reaction |
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