| 不同浓度EGCG对NaCl胁迫下黄瓜种子萌发及其抗性的影响 |
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| 引用本文： | 李洋,刘凯,魏吉鹏,张兰,李鑫,韩文炎,李青云.不同浓度EGCG对NaCl胁迫下黄瓜种子萌发及其抗性的影响[J].浙江农业学报,2018,30(7):1160. |
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| 作者姓名： | 李洋 刘凯 魏吉鹏 张兰 李鑫 韩文炎 李青云 |
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| 作者单位： | 1.河北农业大学 园艺学院,河北 保定 071000;2.中国农业科学院 茶叶研究所,浙江 杭州310008 |
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| 摘 要： | 以黄瓜为实验材料,研究不同浓度表没食子儿茶素没食子酸酯(EGCG)对NaCl胁迫下黄瓜种子萌发、丙二醛(MDA)含量及胚根抗氧化酶活性的影响。结果表明,在非盐胁迫条件下,与对照相比,不同浓度 EGCG(10、100、1 000 μmol·L-1)处理对黄瓜种子萌发、主根伸长及侧根的发生具有一定的浓度效应。在200 mmol·L-1 NaCl处理条件下,对照组黄瓜种子萌发、主根伸长及侧根发生均显著受抑制,同时MDA含量和抗氧化酶活性显著上升;而不同浓度EGCG处理能明显缓解NaCl对黄瓜种子萌发、主根伸长及侧根发生造成的抑制作用,同时降低NaCl胁迫引起的MDA含量,以100 μmol·L-1 EGCG处理效果最佳。测定黄瓜胚根抗氧化酶活性发现,不同浓度EGCG可能通过提高APX、CAT及SOD活性来降低NaCl胁迫下黄瓜的MDA含量。因此,外源添加EGCG可以促进黄瓜种子萌发,可能是通过提高胚根抗氧化酶活性来缓解NaCl引起的根系盐胁迫。
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| 关 键 词： | 表没食子儿茶素没食子酸酯 黄瓜 盐胁迫 种子萌发 丙二醛 抗氧化酶活性 |
| 收稿时间： | 2017-12-10 |
Effects of various concentrations of EGCG on seed germination and resistance in cucumber under NaCl stress |
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| LI Yang,LIU Kai,WEI Jipeng,ZHANG Lan,LI Xin,HAN Wenyan,LI Qingyun.Effects of various concentrations of EGCG on seed germination and resistance in cucumber under NaCl stress[J].Acta Agriculturae Zhejiangensis,2018,30(7):1160. |
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| Authors: | LI Yang LIU Kai WEI Jipeng ZHANG Lan LI Xin HAN Wenyan LI Qingyun |
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| Institution: | 1.Horticulture College, Hebei Agricultural University, Baoding 071000, China;
2. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China |
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| Abstract: | The present study investigated the effects of exogenous application with various concentrations of EGCG (epigallocatechin gallate) on seed germination, malondialdehyde (MDA) content and activities of antioxidant enzymes in radicles of cucumber under NaCl stress. Results showed that, without salt stress, seed germination, radicle length and root branch number responded differently to various concentrations of EGCG. While those were significantly restrained under 200 mmol·L-1 NaCl treatment, accompanying the great increases in content of MDA and activities of antioxidant enzymes. Furthermore, application with various concentrations of EGCG(10, 100, 1 000 μmol·L-1)could significantly increase seed germination, radicle length and root branch number under NaCl stress. Moreover, EGCG application decreased MDA content by improving the activities of antioxidant enzymes (APX, CAT, SOD and POD) under NaCl stress. And the treatment with 100 μmol·L-1 EGCG performed best. These results suggested that exogenous EGCG could enhance seed germination and mitigate NaCl-induced salt stress through promoting the activities of antioxidant enzymes in cucumber. |
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| Keywords: | EGCG cucumber NaCl salt stress seed germination MDA antioxidant enzyme activity |
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