| DON暴露对断奶仔猪抗氧化水平、血清生化指标及小肠黏膜形态的影响 |
| |
| 引用本文： | 王希春,张娅菲,储小燕,朱雷,朱电锋,冯士彬,李玉,吴金节.DON暴露对断奶仔猪抗氧化水平、血清生化指标及小肠黏膜形态的影响[J].浙江农业学报,2018,30(10):1655. |
| |
| 作者姓名： | 王希春 张娅菲 储小燕 朱雷 朱电锋 冯士彬 李玉 吴金节 |
| |
| 作者单位： | 安徽农业大学 动物科技学院,安徽 合肥 230036 |
| |
| 基金项目： | 国家自然科学基金(31472250); 安徽省生猪产业技术体系(AHCYJXTX-05); 安徽农业大学研究生创新基金(2018yjs-25) |
| |
| 摘 要： | 旨在研究脱氧雪腐镰刀菌烯醇(DON)暴露对断奶仔猪抗氧化水平、血清生化指标及小肠黏膜形态的影响。选择健康的“杜×长×大”三元杂交断奶仔猪30头,随机分成3组,对照组饲喂基础饲粮,低剂量组和高剂量组分别饲喂1和2 mg·kg-1的DON饲粮,试验期为60 d。试验结束时,所有仔猪采集血液样本,用于检测抗氧化性能、血清生化指标及肠道通透性。每组选5头仔猪进行剖杀,取小肠组织观察病理变化。结果显示,与对照组相比,DON显著降低了断奶仔猪血清谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性(P<0.05),提高了一氧化氮(NO)和丙二醛(MDA)水平(P<0.05),并引起碱性磷酸酶(ALP)、γ-谷氨酰转移酶(γ-GGT)、肌酐(CREA)、α-淀粉酶(α-AMY)、脂肪酶(LIP)、肌酸激酶(CK)活性显著升高(P<0.05),谷草转氨酶(AST)活性显著降低(P<0.05),血清二胺氧化酶(DAO)及D-乳酸(D-LA)含量随DON剂量的增加而显著升高(P<0.05)。低剂量DON可使小肠黏膜发生部分形态改变,而高剂量DON使小肠组织严重受损。试验结果表明,DON暴露可改变断奶仔猪机体抗氧化反应,引起部分生化指标异常,造成肠道黏膜损伤,增加肠道通透性,对仔猪具有一定的毒性作用。
|
| 关 键 词： | 脱氧雪腐镰刀菌烯醇 仔猪 抗氧化 生化指标 小肠黏膜 |
| 收稿时间： | 2018-03-26 |
Effects of deoxynivalenol exposure on antioxidant capacity,serum biochemical indexes and intestinal mucosal morphology in weanling piglets |
| |
| WANG Xichun,ZHANG Yafei,CHU Xiaoyan,ZHU Lei,ZHU Dianfeng,FENG Shibin,LI Yu,WU Jinjie.Effects of deoxynivalenol exposure on antioxidant capacity,serum biochemical indexes and intestinal mucosal morphology in weanling piglets[J].Acta Agriculturae Zhejiangensis,2018,30(10):1655. |
| |
| Authors: | WANG Xichun ZHANG Yafei CHU Xiaoyan ZHU Lei ZHU Dianfeng FENG Shibin LI Yu WU Jinjie |
| |
| Institution: | College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China |
| |
| Abstract: | To investigate the effects of deoxynivalenol (DON) exposure on antioxidant property, serum biochemical indexes and small intestinal mucosal morphology in weanling piglets, thirty healthy weanling piglets of “Duroc × Landrace × Yorkshire” with similar body weight were selected and randomly divided into 3 groups. Piglets in the control group were fed with basal diet, the low-dose group and the high-dose group were fed with 1 mg·kg-1 and 2 mg·kg-1 of DON diets. The trial was conducted for 60 d. At the end of the experiment, blood samples were collected for detecting the anti-oxidation performance, blood biochemical indexes and small intestinal permeability. Meanwhile, five piglets from each group were slaughtered and the small intestine tissues were taken for the observation of pathological changes. The results indicated that, compared with the control group, the activities of serum glutathione peroxidase(GSH-Px), superoxide dimutase(SOD) and catalase(CAT) of DON groups, were decreased significantly (P<0.05), the levels of nitric oxide(NO) and malondialdehyde(MDA) were increased significantly (P<0.05), the levels of alkaline phosphatase(ALP), gamma-glutamy transpeptidase(γ-GGT), creatinine(CREA), alpha-amylase(α-AMY), lipase(LIP) and creatine kinase(CK)were increased significantly (P<0.05), aspartate amino transferase(AST) was decreased significantly(P<0.05). The serum contents of diamine oxidase(DAO) and D-lactic acid(D-LA) were increased significantly with the increasing concentration of DON. The low-dose of DON resulted in a change of small intestinal mucosal morphology. However, high-dose of DON could cause more serious damage to the intestine. These results suggested that DON exposure could change antioxidant reaction and serum biochemical indexes, lead to intestinal mucosal damage and increase intestinal permeability of piglets, indicating that DON could have toxic effects to weanling piglets. |
| |
| Keywords: | deoxynivalenol piglets antioxidant reaction biochemical index intestinal mucosa |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《浙江农业学报》浏览原始摘要信息 |
| 点击此处可从《浙江农业学报》下载免费的PDF全文 |
|
