Analysis of genetic relationships among 22 European barley varieties based on two PCR markers |
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| Authors: | Araceli Díaz-Perales Rosario Linacero Ana M Vázquez |
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| Institution: | (1) Departamento de Genética, Facultad de Biología, Universidad Complutense de Madrid, 28040 Madrid, Spain |
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| Abstract: | Two long primers of 19 (F17) and 20 (F13) nucleotides, respectively,were used in polymerase chain reactions to amplify DNA from differentcultivated barley accessions. These primers can distinguish closely relatedvarieties and, with a unique primer, all the barley accessions analysedshowed a characteristic fingerprint. Sixty per cent and 76% of thefragments generated using F13 and F17, respectively, were polymorphic.The genetic similarity values between accessions were estimated from F13and F17 data. The dendrogram and principal coordinate analysis performedwith F13 data revealed a clear separation of these varieties in accord withtheir pedigree relationships. |
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| Keywords: | DNA markers genetic similarity Hordeum vulgare L pedigree analysis |
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