| 甘露醇和生长抑制剂对文心兰离体保存的影响 |
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| 引用本文： | 叶秀仙,黄敏玲,吴建设,钟淮钦,林兵,罗远华.甘露醇和生长抑制剂对文心兰离体保存的影响[J].福建农业学报,2011,26(1):76-82. |
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| 作者姓名： | 叶秀仙 黄敏玲 吴建设 钟淮钦 林兵 罗远华 |
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| 作者单位： | 福建省农业科学院作物研究所,福建,福州,350013;福建省农业科学院花卉研究中心,福建,福州,350013;福建省特色花卉工程技术研究中心,福建,福州,350013;农业部闽台农作物种质资源重点开放实验室,福建福州350013 |
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| 基金项目： | 国家科技支撑计划课题，福建省科技重大专项，福建省属公益类科研院所基本科研专项，福建省财政专项-福建省农科院科技创新团队建设基金 |
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| 摘 要： | 以文心兰无菌苗为离体保存材料,研究培养基中添加不同浓度甘露醇、矮壮素及脱落酸对文心兰试管苗离体保存的影响.结果表明:在培养温度(24±3)℃,保存前6个月光照强度2 000～2 500 lx,后6个月光照强度500～1 000 lx,光照时间12 h·d-1的培养条件下,MS+6-BA 0.5 mg·L-1+NAA 0...
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| 关 键 词： | 文心兰 离体保存 甘露醇 生长抑制剂 |
Effect of mannitol and growth inhibitor on in vitro preservation of Oncidium |
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| YE Xiu-xian,HUANG Min-ling,WU Jian-she,ZHONG Huai-qin,LIN Bing,LUO Yuan-hua.Effect of mannitol and growth inhibitor on in vitro preservation of Oncidium[J].Fujian Journal of Agricultural Sciences,2011,26(1):76-82. |
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| Authors: | YE Xiu-xian HUANG Min-ling WU Jian-she ZHONG Huai-qin LIN Bing LUO Yuan-hua |
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| Institution: | YE Xiu-xian1,2,3,4,HUANG Min-ling1,WU Jian-she1,ZHONG Huai-qin1,LIN Bing1,LUO Yuan-hua1,4(1.Institute of Crop Sciences,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China,2.Flower Research Center,3.Fujian Engineering Research Center for characteristics Floriculture Fuzhou,4.Key Laboratory of Crop Germplasm Utilization Between Fujian and Taiwan,Ministry of Agriculture,Fujia... |
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| Abstract: | In vitro preservation of sterile Oncidium plantlets with the addition of mannitol,CCC and/or ABA in the culture medium was investigated.The results showed that(MS + 6-BA) 0.5 mg·L-1 + NAA 0.1 mg·L-1 + sugar 30 g·L-1 in the medium supplemented with mannitol 30 g·L-1 or CCC 30 mg·L-1 or ABA 2.0 mg·L-1 could prolong the plantlet’s shelf life to 12 month at(24±3)℃ under the 12h photoperiod culture condition of 2 000-2 500 lx in the first 6 months,and 500-1 000 lx during the next 6 months.Putting the rates of survival,growth recovery,preservation and proliferation,as well as the cost into consideration,the medium supplemented with 30 g·L-1 mannitol and 30 mg·L-1 CCC was deemed to be most desirable.After 12 months in the medium,the plantlet’s survival rate was 76.7%-80%,and the plant’s growth was normal after cultured in a recovering medium. |
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| Keywords: | Oncidium in vitro preservation mannitol growth inhibitor |
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