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| 米糠和豆粕中植酸的提取及含量的测定 | |
| 引用本文： | 张君朝,任传英,黄文功.米糠和豆粕中植酸的提取及含量的测定[J].黑龙江农业科学,2009(2):132-134. |
| 作者姓名： | 张君朝 任传英 黄文功 |
| 作者单位： | 1. 哈高科大豆食品有限责任公司,黑龙江哈尔滨,150078 2. 黑龙江省哈高科营养食品有限公司,黑龙江哈尔滨,150078 3. 黑龙江省农业科学院经济作物研究所,哈尔滨,150086 |
| 摘 要： | 为了提取米糠和豆粕中的植酸并测定其含量，采用阴离子交换树脂提取植酸，测铁分光光度法测定植酸含量。结果表明，影响提取率的主要因素有提取时间、提取温度、树脂类型及分离时的流速。通过正交试验确定了最佳的提取、分离工艺：提取温度45℃、提取时间3h、D201大孔阴离子交换树脂、流速1mL·min^-1，测得米糠中提取植酸含量为70．39mg·g^-1，豆粕中提取植酸含量为21．14mg·g^-1。该提取方法提取率高，测定方法操作简单、准确度较高，适用于农副产品中植酸的提取及含量的定量测定。 |
| 关 键 词： | 豆粕 米糠 植酸含量 阴离子交换树脂 测铁分光光度法 |
Extraction and Determination of Phytic Acid in the Rice Bran and Bean Pulp |
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| ZHANG Jun-zhao,REN Chuan-ying,HUANG Wen-gong.Extraction and Determination of Phytic Acid in the Rice Bran and Bean Pulp[J].Heilongjiang Agricultural Science,2009(2):132-134. | |
| Authors: | ZHANG Jun-zhao REN Chuan-ying HUANG Wen-gong |
| Institution: | 1.Hi-Tech Soybean Food Co.;Ltd;Harbin;Heilongjiang 150078;2.Heilongjiang Hi-Tech Nutriment Food Co.;3.Industrial Crops Institute of Heilongjiang Academy of Agricultural Sciences;Heilongjiang 150086 |
| Abstract: | Anion exchange resin was used to extract phytic acid and measure iron pectrophotometric method was adopted to determine phytic acid concentration. The results showed that： Extraction time, extraction temperature, resin type and flow rate were main influencing factors. The optimal craft were that extraction temperature was 45℃, extraction time was 3 h, D201 anion exchange resin was adapted to disassociation, flow rate was i mL per rain. Phytic acid content were 70.39 mg·g^-1 and 21.14 mg·g^-1 by the method in the rice and bean pulp respectively. It could be concluded that the extraction method was effective and the assay method was simple ,accurate,they were adapt to extraction and determine the phytic acid in the agriculture by-product. |
| Keywords: | bean pulp rice bran the content of phytic acid anion exchange resin measure iron pectrophotometric method |
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