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| 红魔芋不同外植体不定芽诱导的比较研究 | |
| 引用本文： | 普丽花,陈燕萍,卢丽英,李宁,玉尖章,何立群,秦琪书,谢庆华.红魔芋不同外植体不定芽诱导的比较研究[J].中国园艺文摘,2010,26(10):22-23,38. |
| 作者姓名： | 普丽花 陈燕萍 卢丽英 李宁 玉尖章 何立群 秦琪书 谢庆华 |
| 作者单位： | 1. 云南省西双版纳州农业科学研究所,云南,景洪,666100 2. 昆明琪鼎权生物科技有限公司,云南,昆明,650035 3. 云南师范大学生物资源研究所,云南,昆明,650092 |
| 基金项目： | 云南省农业科技示范推广项目(云财计)46号 |
| 摘 要： | 红魔芋是自然界中含有大量葡甘聚糖的经济作物之一，其繁殖系数较低，病毒感染及种质退化严重。为解决红魔芋繁殖系数低、种质退化等问题，以红魔芋的球茎和无性株茅为外植体进行组织培养试验。研究表明，地下球茎是最佳外植体；75％的酒精浸泡30s，再用0．15％的升汞浸泡30min是最理想的消毒方式；愈伤及不定芽最佳培养基为MS＋6-BA2mg／L＋NAA0．1mg／L。 |
| 关 键 词： | 红魔芋 地下球茎 无性株芽 不定芽 |
Comparative Study on the Adventitious Shoots Inducing Different Explants in Vitro in A.xiei |
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| Authors: | Pu Li-hua Chen Yan-ping Lu Li-ying Li Ning Yu Jian-zhang HE Li-qun Qin Qi-shu Xie Qing-Hua |
| Institution: | Pu Li-hua Chen Yan-ping Lu Li-ying Li Ning Yu Jian-zhang HE Li-qun Qin Qi-shu Xie Qing-Hua |
| Abstract: | A.xiei is one economic crops with rich-konjac glucomannan in nature, but its propagation was lower, and the plant virus infection and germplasm degradation was severe. To solve the problem of low propagation coefficient and germplasm degradation in A. xiei, two exptants： bulbs and bulbils, were used for propagation in vitro in tissue culture in A.xiei. The results show that the underground bulb is the best explants; the best way to disinfect is to soak the explant in 0.15% mercuric chloride for 30minutes after soaking it in 75% alcohol for 30 seconds; the best medium for inducing callus and bud is： MS＋6-BA2mg/L＋NAA0.1mg/L. |
| Keywords: | A xiei underground bulbs bulbils adventitious buds |
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