| 多抗性保加利亚乳杆菌的诱变和筛选 |
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| 引用本文： | 芦烨,梁金钟,施铜铃.多抗性保加利亚乳杆菌的诱变和筛选[J].乳业科学与技术,2010,33(1):6-10. |
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| 作者姓名： | 芦烨 梁金钟 施铜铃 |
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| 作者单位： | 哈尔滨商业大学食品工程学院,黑龙江哈尔滨,150076 |
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| 基金项目： | 课题为哈尔滨市"十一五"科技攻关项目 |
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| 摘 要： | 通过紫外诱变和硫酸二乙酯诱变,筛选出抗生素抗性(头孢氨苄和氨苄西林)的保加利亚乳杆菌,同时模拟人体胃酸环境(pH1.5～4.5)和十二指肠高胆酸盐环境(0.1g/100mL～0.4g/100mL)对诱变后的保加利亚乳杆菌的抗性进行了研究。结果表明,保加利亚乳杆菌突变株可抗头孢氨苄和氨苄西林(均为2mg/mL),并且在pH2.5～4.5时具有较强的生存能力,培养4h活菌数仍达108cfu/mL以上,在pH1.5条件下仍有部分存活。在0.1g/100mL～0.3g/100mL胆酸盐条件下培养4h,活菌数仍达106cfu/mL以上,且能在0.4g/100mL胆汁盐中存活。
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| 关 键 词： | 乳酸菌 诱变 抗生素抗性 胃酸胆盐 |
Selection of Multi-resistance Lactobacillus bulgaricus Strain |
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| Lu Ye,Liang Jinzhong,Shi Tongling.Selection of Multi-resistance Lactobacillus bulgaricus Strain[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2010,33(1):6-10. |
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| Authors: | Lu Ye Liang Jinzhong Shi Tongling |
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| Institution: | Lu Ye,Liang Jinzhong,Shi Tongling Food Engineering College of Harbin University of Commerce,Harbin of Heilongjiang 150076,China |
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| Abstract: | Lactobacillus bulgaricus strain which was anti-antibiotic （Cephalexin and Ampicillin）, was selected by UV mutagenesis and DES mutagenesis. The resistance ability of the mutant was studied by the simulated gastric acid （pH 1.5-4.5） and duodenal bile salt environment （0.1 g/100mL-0.4 g/100mL） in vitro. The results indicated that the mutant could survive at 2 mg/mL Cephalexin or Ampicillin, and the mutant also could survive at pH 1.5 and viable bacterial counts reached 10^8 cfu/mL at pH 2.5-4.5 after 4 h incubation. Furthermore, the mutant could survive at 0.4 g/100mL bile salt and viable bacterial counts reached 10^8 cfu/mL at 0.1 g/100mL-0.3 g/100mL bile salt after 4 h incubation. |
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| Keywords: | Lactobacillus mutation anti-antibiotic gastric acid bile salt |
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