| 蝴蝶兰叶片诱导类原球茎的研究 |
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| 引用本文： | 张雯頩,张晓波,潘刚,刘宪虎.蝴蝶兰叶片诱导类原球茎的研究[J].湖北农业科学,2010,49(5). |
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| 作者姓名： | 张雯頩 张晓波 潘刚 刘宪虎 |
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| 作者单位： | 1. 延边大学农学院,吉林,龙井,133400
2. 浙江大学农业与生物技术学院,杭州,310029 |
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| 摘 要： | 以14个蝴蝶兰(Phalaenopsis amabilis)品种无菌苗叶片为外植体诱导类原球茎(Protocorn-likebody,PLB)。结果表明,在培养基1/2MS+3mg/L TDZ+20g/L蔗糖+3g/L植物凝胶上,14个蝴蝶兰品种的叶片均能诱导出类原球茎,其中10个品种的诱导率高于20%,尤以777最高,为51%;最低的为788,仅有1%。试验建立了一套成本低廉、成分简单、重复性好、也适合于蝴蝶兰类原球茎的继代增殖培养技术体系,为蝴蝶兰的快速发展创造了条件。
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| 关 键 词： | 蝴蝶兰 叶片 类原球茎 |
Study on Protocorn-like Body Induced from the Leaves of Phalaenopsis amabilis |
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| ZHANG Wen-ping,ZHANG Xiao-bo,PAN Gang,LIU Xian-hu.Study on Protocorn-like Body Induced from the Leaves of Phalaenopsis amabilis[J].Hubei Agricultural Sciences,2010,49(5). |
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| Authors: | ZHANG Wen-ping ZHANG Xiao-bo PAN Gang LIU Xian-hu |
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| Institution: | ZHANG Wen-ping1,ZHANG Xiao-bo2,PAN Gang2,LIU Xian-hu1 (1. College of Agriculture,Yanbian University,Longjing 133400,Jilin,China,2. College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029,China) |
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| Abstract: | The protocorn-like body was induced from leaves of fourteen varieties Phalaenopsis amabilis species by the same media. The results showed that the suitable medium for protocorn-like body inducement from leaves of fourteen varieties of P. amabilis was 1 / 2MS + 3mg / L TDZ + 20g / L sucrose + 3g / L phytagel. The induction rates of fourteen varieties were: V31:32%,8106:33%,729:46%,777:51%,604:21%,602-3:9%,791:28%,8169:24%,735:31%,607:21%,771:4%,608:38%, 728-3:18%,788:1%. The medium was low-cost,simple compos... |
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| Keywords: | Phalaenopsis amabilis leaf protocorn-like body |
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