Study on the stability and reusability of Glutamate Dehydrogenase immobilized on bacterial cellulose nanofiber |
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| Authors: | M Pesaran Gh Amoabediny |
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| Institution: | 1.Department of Life Science Engineering, Faculty of New Sciences and Technologies,University of Tehran,Tehran,Iran;2.Department of Chemical Engineering, Faculty of Engineering,University of Tehran,Tehran,Iran;3.Department of Bioreactor Engineering, Research Center for New Technologies in Life Science Engineering,University of Tehran,Tehran,Iran |
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| Abstract: | In this study, a simple method with improved properties for Glutamate Dehydrogenase immobilization is proposed by using bacterial cellulosic nanofiberas as a novel and inexpensive biomatrix. Acetobacter xylinum ATCC 10245 has been chosen and cultivated for synthesis of this nanofiber with the diameter of 30-70 nm. Thereafter, Glutamate Dehydrogenase was immobilized into the nanofiber pores in two steps: 1) enzyme adsorption and 2) crosslinking by glutaraldehyde. Optimum pH for the activity of immobilized enzyme and optimum temperature was reported in this work. Also the relative activity of immobilized enzyme was about 38 % in comparison to the enzyme activity of the first day after about two months. Moreover after reusing of the enzyme for 9 cycles, the immobilized enzyme retained over 83 % of its initial activity. Glutamate Dehydrogenase is immobilized on bacterial cellulosic nanofiber for the first time which can affect on facilitation of Glutamate production. Also can be a good method for having a stable and robust immobilized Glutamate Dehydrogenase that can withstand harsh operating conditions in processes which are common in these kind of reactions. |
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