| 番茄叶霉病菌对氟硅唑敏感基线及抗药性风险研究 |
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| 引用本文： | 祁之秋,纪明山,张弛,孙晶,程根武,谷祖敏,王英姿.番茄叶霉病菌对氟硅唑敏感基线及抗药性风险研究[J].植物保护,2004,30(1):53-55. |
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| 作者姓名： | 祁之秋 纪明山 张弛 孙晶 程根武 谷祖敏 王英姿 |
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| 作者单位： | 沈阳农业大学植物保护学院农药科学系,沈阳,110161 |
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| 摘 要： | 从辽宁省不同地区未使用过氟硅唑的保护地中采集番茄叶霉病叶,经单孢分离获得43株叶霉病菌。采用茵丝生长速率法测定其对氟硅唑的敏感性。结果表明,菌株间存在较大差异,EC50值最低为0.151 9μg／mL,最高达4.647 5 μg／mL。所有菌株的EC50总平均值为1.253 1μg/mL,可作为番茄叶霉病菌对氟硅唑的敏感基线。野生菌株经紫外线照射,获得两个抗药性突变菌株,其抗药性倍数分别为6.0和7.6。突变菌株的菌丝生长速率及产孢能力显著低于原野生菌株。
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| 关 键 词： | 植物病理学 番茄叶霉病菌 氟硅唑 敏感基线 抗药性 |
Baseline sensitivity of Fulvia fulva on tomato to flusilazole and resistance risk analysis |
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| QI Zhi-qiu;JI Ming-shan;ZHANG Chi;SUN Jing;CHENG Gen-wu;GU Zu-min;WANG Ying-zi.Baseline sensitivity of Fulvia fulva on tomato to flusilazole and resistance risk analysis[J].Plant Protection,2004,30(1):53-55. |
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| Authors: | QI Zhi-qiu;JI Ming-shan;ZHANG Chi;SUN Jing;CHENG Gen-wu;GU Zu-min;WANG Ying-zi |
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| Institution: | Department of Pesticide Science;College of Plant Protection;Shenyang Agricultural University;Shenyang 110161;China |
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| Abstract: | Infacted tomato leaves were collected from protected fields without exposure to flusioazole in different regions of Liaoning Province to obtain 43 Fulvia fulva wild-type single-spore isolates. The baseline sensitivity was determined by measuring mycelial growth rate. The results showed that the baseline sensitivity of 43 isolates ranged from 0.151 9 μg/mL to 4.647 5 μg/mL. The mean EC50 value was 1.235 1 μg/mL. Two UV mutants with 6.0 and 7.6 resistance times to flusilazole from a wild - type isolate were obtained. The mutants also showed reduced growth and sporulation. The results indicated that the resistance risk of Fulvia fulva to flusilazole was low. |
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| Keywords: | phytopathology tomato leaf mold flusilazole baseline sensitivity fungicide resistance |
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