| 从海南省杂草胜红蓟和假马鞭上检测到粉虱传双生病毒 |
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| 引用本文： | 范三薇,周雪平.从海南省杂草胜红蓟和假马鞭上检测到粉虱传双生病毒[J].植物病理学报,2003,33(6):513-516. |
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| 作者姓名： | 范三薇 周雪平 |
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| 作者单位： | 浙江大学生物技术研究所, 杭州 310029 |
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| 基金项目： | 国家杰出青年基金(30125032) |
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| 摘 要： | 利用三抗体夹心ELISA(TAS-ELISA)及PCR检测的方法对采自海南的胜红蓟(Ageratum conyzoides)和假马鞭(Stachytarphetajamaicensis)的5个病样进行了检测,表明均为粉虱传双生病毒。PCR扩增产物克隆后进行序列测定,结果表明存在2类双生病毒,其中样品Hn2存在2类病毒的复合侵染。这是在海南首次报道存在有粉虱传双生病毒。
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| 关 键 词： | 粉虱传双生病毒 三抗体夹心ELISA PCR |
| 文章编号： | 0412-0914(2003)06-0513-04 |
| 修稿时间： | 2003年3月31日 |
Detection of whitefly-transmitted geminivirus in Ageratum conyzoides and Stachytarpheta jamaicensis in Hainan, China |
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| FAN San-wei,ZHOU Xue-ping Institute of Biotechnology,Zhejiang University,Hangzhou ,China.Detection of whitefly-transmitted geminivirus in Ageratum conyzoides and Stachytarpheta jamaicensis in Hainan, China[J].Acta Phytopathologica Sinica,2003,33(6):513-516. |
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| Authors: | FAN San-wei ZHOU Xue-ping Institute of Biotechnology Zhejiang University Hangzhou China |
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| Institution: | Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China |
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| Abstract: | Five virus samples collected from Ageratum conyzoides and Stachytarpheta jamaicensis in Hainan were detected
with triple antibody sandwich ELISA (TAS-ELISA) and PCR, and the results showed that all the virus samples were infec-
ted by whitefly-transmitted geminiviruses. Sequence analyses showed two types of viruses existed among those samples, and
sample Hn2 was mix-infected by two types of viruses. This is Ihe first report of whitefly-transmitted geminiviruses in Hainan
Province. |
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| Keywords: | whitefly-transmitted geminiviruses TAS-ELISA PCR |
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