|
|||||
|
|
Small ruminant lentiviruses in Jordan: evaluation of sheep and goat serological response using recombinant and peptide antigens |
|
| Authors: | Francesco Tolari Wafa’a Al-Ramadneh Maurizio Mazzei Maria Luisa Carrozza Mario Forzan Patrizia Bandecchi Elena Grego Sergio Rosati |
| Institution: | 1. Dipartimento di Scienze Veterinarie, University of Pisa, Viale delle Piagge, 2, 56124, Pisa, Italy 2. International Cooperation Division, Ministry of Agriculture, Amman, The Hashemite Kingdom of Jordan 3. Scuola Normale Superiore, Piazza dei Cavalieri 7, 56126, Pisa, Italy 4. Dipartimento di Produzioni Animali Epidemiologia ed Ecologia, Universita di Torino, Via L. Da Vinci 44, 10044, Turin, Italy |
| Abstract: | Small ruminant lentiviruses infect sheep and goats worldwide, causing chronic progressive diseases and relevant economic losses. Disease eradication and prevention is mostly based on serological testing. The goal of this research was to investigate the presence of the small ruminant lentiviruses (SRLVs) in Jordan and to characterize the serological response in sheep and goat populations. A panel of sera were collected from flocks located in Northern Jordan and Jordan Valley. The samples were tested using three ELISA assays: a commercially available ELISA based on p25 recombinant protein and transmembrane peptide derived from British maedi–visna virus (MVV) EV1 strain, an ELISA based on P16-P25 recombinant protein derived from two Italian strains representative of MVV- and caprine arthritis encephalitis virus (CAEV)-like SRLVs, and an ELISA based on SU5 peptide from the same two Italian isolates. The results indicate that both MVV- and CAEV-like strains are present in Jordan and that the majority of the viruses circulating among sheep and goat populations belong to the MVV-like genotype. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |