| 新银合欢中单宁提取工艺优化研究 |
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| 引用本文： | 李茂,字学娟,周汉林,刘国道.新银合欢中单宁提取工艺优化研究[J].草业科学,2012,29(2):301-305. |
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| 作者姓名： | 李茂 字学娟 周汉林 刘国道 |
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| 作者单位： | 中国热带农业科学院热带作物品种资源研究所,海南儋州571737／中国热带农业科学院热带草业与畜牧研究所,海南儋州571737;中国热带农业科学院热带作物品种资源研究所,海南儋州571737／中国热带农业科学院热带草业与畜牧研究所,海南儋州571737／海南大学应用科技学院（儋州校区）,海南儋州571737 |
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| 基金项目： | 中央级公益科研院所基本科研业务专项，海南省重点科技项目，农业科技成果转化资金项目 |
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| 摘 要： | 对新银合欢（Leucaena leucocephala）中单宁类物质的提取工艺进行优化研究，选择液料比、提取温度、提取时间、提取剂体积分数等因素分别进行单因素和正交试验。结果表明，在液料比40∶1、提取温度50 ℃、提取时间60 min、丙酮体积分数50%的条件下，单宁提取效果最佳，测得新银合欢单宁平均含量为2.54%。
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| 关 键 词： | 新银合欢 单宁 提取 |
Optimum conditions on extraction of tannin from Leucaena leucocephala |
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| LI Mao,ZI Xue-juan,ZHOU Han-lin,LIU Guo-dao.Optimum conditions on extraction of tannin from Leucaena leucocephala[J].Pratacultural Science,2012,29(2):301-305. |
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| Authors: | LI Mao ZI Xue-juan ZHOU Han-lin LIU Guo-dao |
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| Institution: | 1,2(1.Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737,China;2.Tropical Grassland and Animal Science Institute, Chinese Academy of Tropical Agricultural Sciences,Danzhou 571737,China; 3.College of Applied Science and Technology,Hainan University,Danzhou 571737,China) |
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| Abstract: | The optimum conditions,including four main factors: ratio of liquid to material,extraction temperature,extraction time and extractant concentration,for extracting tannins from leaves and stems of Leucaena leucocephala were optimized by using a combined method of single factor analysis and orthogonal experiment design.The results showed that the optimum conditions of each factor in tannin extraction were: ratio of liquid to material 40∶1,extraction temperature 50 ℃,extraction time 40 min and acetone concentration 50%.The extracting rate of the tannin from the leaves and stems of L.leucocephala was 2.54%. |
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| Keywords: | Leucaena leucocephala tannins extraction |
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