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粗皮侧耳粗毒素的提取及杀线虫活性的研究
引用本文:向红琼,冯志新.粗皮侧耳粗毒素的提取及杀线虫活性的研究[J].云南农业大学学报,1999,14(Z1):82-87.
作者姓名:向红琼  冯志新
作者单位:华南农业大学植物线虫研究室!广州510642
摘    要: 粗皮侧耳(Pleurotus ostreatus)菌物能产生杀线虫毒素.用乙酸乙酯从该菌丝麦粒培养物中提取的粗毒素具有杀线虫活性,稀释10倍,击倒95%的线虫(Panagrellus redivivus)仅需4.5h.该菌产生的毒素可溶于水及各种有机溶剂, 对热具有高度的稳定性,对线虫的活性具有一定的广谱性.

关 键 词:粗皮侧耳  杀线虫粗毒素  粗毒素基本特性
收稿时间:1999-05-06

Nematicitdal Activity and Elementary Characteristics of the Crude from Pleurotus ostreatus
Xiang Hongqiong,Feng Zhixin.Nematicitdal Activity and Elementary Characteristics of the Crude from Pleurotus ostreatus[J].Journal of Yunnan Agricultural University,1999,14(Z1):82-87.
Authors:Xiang Hongqiong  Feng Zhixin
Institution:Laboratory of Plant Nematology, South China Agri. Univ. Guangzhou 510642
Abstract:Pleurotus ostreatus is a commone boasidiomyct in the world.The hyphae of Pleurotus ostreatus can produce nematotoxin. The nematicidal activity compenent was detected in the ethyl acetate crude extract from P osrteatus hyphae cultured on moistened,autoclaved wheat.When thecrude PO-toxin was diluted 10X,95% of nematodes were immobilizied within 4 h and 30 minutes.The nematotoxin of P ostreatus dissolves in water and in acetone,ethyl acetate,methanol,but the nematicidal activity was not detected in the Petroleum ether.It had activity to many nematodes, such as Hirschmanniella oryzae, Bursaphelenchus xylophilus, Pratylenshus sp., Meloidogyne incognita, M.arenaria and so on.
Keywords:Pleurotus ostreatus  Nematicidal activity of crude PO-toxin  Characteristics of crude PO-toxin
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