| 适用于转录组测序的棉花幼根总RNA提取方法筛选 |
| |
| 引用本文： | 王春晖,赵云雷,王红梅,陈伟,龚海燕,桑晓慧.适用于转录组测序的棉花幼根总RNA提取方法筛选[J].棉花学报,2013,25(4):372-376. |
| |
| 作者姓名： | 王春晖 赵云雷 王红梅 陈伟 龚海燕 桑晓慧 |
| |
| 作者单位： | 中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳,455000 |
| |
| 基金项目： | 国家自然科学基金，2013年中央级公益性科研院所基本科研业务专项 |
| |
| 摘 要： | 棉花幼根中含有大量酚类物质、多糖和单宁等多种次生代谢物,且根部取样时会带有少量的泥土和有机质等污染物,因此棉花幼根RNA提取难度较大.本文采用两种试剂盒法和改良的异硫氰酸胍法,筛选适合转录组测序的提取棉花幼根RNA的方法.与试剂盒法相比,改良的异硫氰酸胍法具有浓度高、质量好等特点,能够满足转录组测序的要求.
|
| 关 键 词： | 棉花 转录组 RNA提取方法 根部 |
| 收稿时间： | 2013-01-28 |
Screening of Methods to Isolate High-quality Total RNA from Young Cotton Roots |
| |
| WANG Chun-hui
,
ZHAO Yun-lei
,
WANG Hong-mei
,
CHEN Wei
,
GONG Hai-yan
,
SANG Xiao-hui.Screening of Methods to Isolate High-quality Total RNA from Young Cotton Roots[J].Cotton Science,2013,25(4):372-376. |
| |
| Authors: | WANG Chun-hui ZHAO Yun-lei WANG Hong-mei CHEN Wei GONG Hai-yan SANG Xiao-hui |
| |
| Institution: | Institute of Cotton Research of CAAS/State Key Laboratory of Cotton Biology, Anyang, Henan4 55000, China |
| |
| Abstract: | It is difficult to obtain high-quality RNA from young cotton roots, because the root tissues contain large amounts of various secondary metabolites, including polyphenolics, polysaccharides, and tannins. Also, soil and other organic contaminants are often mixed with the roots when sampling. In this study, two commercial kits and an improved guanidine thiocyanate method were screened as methods to isolate total RNA from young cotton roots, with the aim of producing RNA of sufficient quality for RNA-sequencing analyses. The results showed that compared with the plant RNAout kits, the improved guanidine thiocyanate method resulted in greater quantities of RNA, and higher RNA quality. The RNA obtained using this method was suitable for RNA-sequencing analyses. |
| |
| Keywords: | cotton RNA-seq RNA extraction method cotton root |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《棉花学报》浏览原始摘要信息 |
| 点击此处可从《棉花学报》下载免费的PDF全文 |
|
