Immuno-histological mapping and functional association of seminal proteins in testis and excurrent ducts with sperm function in buffalo |
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| Authors: | Sunil Kumar Patil Lakshminarayana Somashekar Sellappan Selvaraju Kolatalu V Jamuna Shivashanmugam Parthipan Bala Krishnan Binsila Ramaswamy Venkatesha Prasad Janivara Parameshwaraiah Ravindra |
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| Institution: | 1. Reproductive Physiology Laboratory, Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, India
Department of Veterinary Anatomy and Histology, Veterinary College, KVAFSU, Bengaluru, India;2. Reproductive Physiology Laboratory, Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, India;3. Department of Veterinary Anatomy and Histology, Veterinary College, KVAFSU, Bengaluru, India |
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| Abstract: | The region-specific expression of seminal proteins in testis and excurrent duct system determines the quality and function of the spermatozoa. In the present study, localization and expression of some of the seminal proteins such as insulin-like growth factor receptor 1β (IGF-1Rβ), phosphatidylethanolamine-binding protein 4 (PEBP4), α-tubulin and tissue factor pathway inhibitor 2 (TFPI2) were carried out in testis, excurrent duct system and spermatozoa of buffalo. IGF-1Rβ was localized in the cells of the seminiferous tubules of the testis, except in primary spermatocytes. The PEBP4 was localized only in the elongated spermatid, whereas α-tubulin and TFPI2 proteins were localized in all cells of the seminiferous tubule including spermatocyte. In the buffalo spermatozoa, IGF-1Rβ, PEBP4, α-tubulin and TFPI2 were localized in the acrosome region, the post-acrosomal region till the tail end, post-acrosome to the entire tail region and the equatorial region, respectively. The study indicates that IGF-1R, α-tubulin and PEBP4 proteins regulate spermatogenesis, whereas TFPI2 may be involved during the zona binding process of the buffalo spermatozoa. |
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| Keywords: | buffalo excurrent duct system localization and expression seminal proteins sperm function testis |
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