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Tetraploid inductions of Penaeus monodon using cold shock |
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| Authors: | Andrew R Foote Graham C Mair Andrew T Wood Melony J Sellars |
| Institution: | 1. CSIRO Food Futures National Research Flagship, CSIRO Marine and Atmospheric Research, EcoSciences Precinct, Dutton Park, QLD, 4102, Australia 2. School of Biological Sciences, Flinders University, Sturt Road, Bedford Park, Adelaide, SA, 5042, Australia 3. CSIRO Marine and Atmospheric Research, GPO Box 2583, Brisbane, QLD, 4001, Australia |
| Abstract: | Genetic protection of improved lines of Giant Tiger shrimp, Penaeus monodon, may be achieved through the reliable production of all triploid families, as triploid shrimp are reproductively sterile. The problem with current triploid induction methods is in producing 100?% triploid progeny reliably and doing so on a commercial scale. All triploid offspring may be achievable by mating tetraploids with diploids, overcoming current limitations. However, tetraploidy in shrimp to date has been unsuccessful, with all tetraploids dying very early in development. As the induction method of heat was found to be lethal, this study investigated an alternative method using cold water to assess induction rates and lethality. Cold-water treatments between ?2 and 14?°C were applied at 18, 20, and 22?min post-spawning detection for various durations with the aim of inhibiting first mitosis. Tetraploidy was induced in treatments at or less than 1?°C; however, no nauplii hatched from these treatments. As no diploid or tetraploid nauplii hatched from any of the treatments below 3?°C, it was determined that this cold-water treatment method is not suitable for tetraploid production in P. monodon. |
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