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Effects of estradiol-17β and 17α-methyltestosterone on gonadal differentiation in the coho salmon, Oncorhynchus kisutch
Authors:Frederick W Goetz  Edward M Donaldson  George A Hunter  Helen M Dye
Institution:1. Resource Services Branch, Department of Fisheries and Oceans, 4160 Marine Drive, West Vancouver, B.C. V7V 1N6 Canada;1. University of Notre Dame, Department of Biology, Notre Dame, Ind. 46556 U.S.A.
Abstract:Methods are described for controlling sex differentiation in the coho salmon, Oncorhynchus kisutch. Eyed eggs and alevins were immersed in solutions of estradiol-17β and 17α-methyltestosterone and fed these steroids in the diet for a period of 10 weeks postswim-up. In seven out of 10 groups that received estradiol all fish resembled normal females when sampled at 4 months posthatch. In fish treated with methyltestosterone at all except the lowest dose (25 and 50 μg/l) the gonads resembled neither normal males nor normal females; these gonads were composed largely of connective tissue with only occasional germ cells. Various proportions of male, female and intersex gonads were observed in groups which received androgen or estrogen only in the diet. The implications of the work for salmon culture include the production of sterile fish and increasing the proportion of female salmon in hatchery populations.
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