| 高效液相色谱-荧光/紫外串联测定
海洋沉积物中16种多环芳烃 |
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| 作者姓名： | 杨帆 翟毓秀 任丹丹 郭萌萌 吴海燕 谭志军 |
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| 作者单位： | [1]大连海洋大学,116023 [2]农业部水产品质量安全检测与评价重点实验室国家水产品质量监督检验中心中国水产科学研究院黄海水产研究所,青岛266071 |
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| 基金项目： | 国家自然科学基金项目（41106109）和中央级基本科研业务费项目（20603022012019;20603022011004）共同资助 |
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| 摘 要： | 采用高效液相色谱-荧光和紫外检测联用技术,建立了海洋沉积物中16种多环芳烃（PAHs）的同时测定方法.样品用异丙醇提取,HLB固相萃取柱净化,Waters PAHs C18 （4.6 mm×250 mm,5μm）色谱柱分离,乙腈-水溶液梯度洗脱,荧光-紫外检测器串联检测.本方法中,16种PAHs在各自相应浓度范围内线性良好,相关系数均大于0.999.添加水平为5μg/kg和10 μg/kg时,回收率在72.6％～117％之间（n=6）,RSD％均小于10％,检出限（S/N=3）为0.2～4.9 μg/kg.利用该方法对采自大连和烟台沿岸的沉积物样品进行了分析,发现均含有一定的PAHs组分.该方法方便、快捷、灵敏度高,为海洋沉积物中PAHs的污染分析及风险评估提供了良好的方法基础.
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| 关 键 词： | 高效液相色谱 荧光-紫外检测器联用 海洋沉积物PAHs |
| 收稿时间： | 2012/10/30 0:00:00 |
| 修稿时间： | 2013/1/21 0:00:00 |
Determination of sixteen polycyclic aromatic hydrocarbons
in marine sediments by high performance liquid chromatography
with fluorescence and ultraviolet detection |
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| Authors: | YANG Fan ZHAI Yu-xiu GUO Meng-meng WU Hai-yan REN Dan-dan TAN Zhi-jun |
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| Institution: | 1 Dalian Ocean University, 116023)(2 Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture National Center for Quality Supervision and Test of Aquatic Products, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071) |
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| Abstract: | An analytical method based on liquid phase of fluorescence detector combined with UV detector has been developed for the simultaneous determination of sixteen polycyclic aromatic hydrocarbons （PAHs） in marine sediments.After being extracted with isopropanol,the extract was distilled with HLB column,then performed on HPLC with a Waters PAH C18 column （4.6 mm× 250 mm,5 μm）,separated by gradient elution using a mixture of acetonitrile and water as mobile phase.The calibration curves were linear with correlation coefficient over 0.999.The average recoveries from spiked marine sediments at the 5μg/kg and 10μg/kg concentrations ranged from 72.6 % ～117 % （n=6） with relative standard deviations less than 10 %,and limits of detection（LOD） were 0.2～4.9μg/kg.Some PAHs were found in six marine sediment samples collected from Dalian and Yantai areas.This method was validated to be accurate and sensitive for the analysis and risk assessment of PAHs in marine sediments. |
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| Keywords: | HPLC Fluorescence-UV Marine sediments PAHs |
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