Isolation and structural characterization of rhamnogalacturonan II-borate complex fromPinus densiflora |
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| Authors: | Tomoko Shimokawa Tadashi Ishii Toshiro Matsunaga |
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| Institution: | (1) Forest and Forest Products Research Institute, PO Box 16, 305-8687 Tsukuba Norm, Kenkyu Danchinai, Ibaraki, Japan;(2) Kyushu National Agricultural Experiment Station, 861-1192 Nichigoshi, Kumamoto, Japan |
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| Abstract: | A boron-containing pectic polysaccharide was isolated from Driselase digests of akamatsu (Pinus densiflora) cell wall. The boron-containing polysaccharide was purified by successive column chromatography of Cosmosil C18 OPN, DEAE Sepharose FF, and Superdex 75. The complex had 0.12% (w/w) boron (B) and11B-NMR spectroscopy showed the complex to be the tetrahedral diester form of boron. The sugar composition and glycosyl linkage analyses showed that the sugar portion of the complex was rhamnogalacturonan-II (RG-11). The boron-attached glycosyl residue was identified to be 2-O-Me-xylose-containing apiosyl side chain in RG-II. These results established that the location of borate in the cell wall is conserved among dicots, gramineous monocots, and coniferous gymnosperm.Part of this study was presented at the 8th Cell Wall Symposium, Norwich, September 3, 1998 |
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| Keywords: | Pinus densiflora Gymnosperm Pectin Rhamnogalacturonan II Boron |
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