Detecting<Emphasis Type="Italic">Orobanche</Emphasis> species by using cpDNA diagnostic markers |
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| Authors: | B Román C I González Verdejo Z Satovic M D Madrid J I Cubero S Nadal |
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| Institution: | (1) CIFA, Alameda del Obispo, IFAPA-CICE, 14080 Córdoba, Spain;(2) Dept. of Seed Science and Technology, Faculty of Agriculture, University of Zagreb, 10000 Zagreb, Croatia;(3) Depto. de Genética, ETSIAM, Universidad de Córdoba, 14071 Córdoba, Spain |
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| Abstract: | Some species of the genusOrobanche are among the most devastating parasitic weeds, causing extensive damage in agricultural fields. Considering the difficult
control due to seed longevity in the soil, small seed size, high fecundity and a subterranean phase that allows them to parasitize
the host before they emerge and become evident, the development of diagnostic markers is highly recommended. In our study
we identified potential molecular diagnostic markers from the plastid genome in order to distinguish among the most importantOrobanche species attacking crops in Andalusia, the southern region of the Iberian Peninsula. The study has consideredO. crenata, O ramosa andO. cumana causing serious losses in legumes, solanaceous crops and sunflower fields, respectively, andO. minor that, although abundant in Andalusia, has to our knowledge not yet been found parasitizing agricultural hosts. We amplified
a non-coding region from the plastid genome, studied sequence differences among the amplified fragments and digested those
of the same length with selected restriction enzymes. Here, we propose a molecular protocol to distinguish the main parasitic
plants in crop fields of southern Spain. Different applications such as identification ofOrobanche seeds in soil or crop seed lots are discussed in order to offer right crop recommendations or to prevent new infestation
of parasite-free fields. Recommendations for further development of these diagnostic markers are also considered.
http://www.phytoparasitica.org posting Jan. 15, 2007. |
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| Keywords: | Orobanche diagnostic markers plastid DNA agricultural fields Andalusia |
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