| Wpkci基因用于鸡与鹌鹑属间杂交早期胚胎性别鉴定 |
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| 引用本文： | 乔爱君,马文霞,李大全,孟庆美.Wpkci基因用于鸡与鹌鹑属间杂交早期胚胎性别鉴定[J].中国农业科学,2008,41(3):841-845. |
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| 作者姓名： | 乔爱君 马文霞 李大全 孟庆美 |
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| 作者单位： | 新疆石河子大学动物科技学院,新疆石河子,832003 |
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| 摘 要： | 【目的】建立鉴定鸡（♂）与鹌鹑（♀）属间杂交早期胚胎性别的方法，检验早期胚胎性别比。【方法】人工授精获得鸡与鹌鹑杂交种蛋，按照鸡的标准孵化条件同批入孵，连续随机采集发育早期66、72、78、84、90、96、102、108、114、120 h活胚。采用RT-PCR方法，以已知性别的鹌鹑作外参照，β-actin引物作内参照，用鹌鹑Wpkci基因保守区设计的引物鉴定早期胚胎样本性别。【结果】Wpkci引物可以准确鉴定杂交胚胎的性别；早期胚胎性比率与理论值相比，差异极显著（P＜0.01），雄性明显多于雌性；不同天数间性别比率差异不显著（P＞0.05）。【结论】利用Wpkci引物建立了一种简单、快速、可靠、稳定的鉴别杂交胚胎性别的方法；早期胚胎雌性死亡率明显高于雄性，雌性死亡率无特定高峰，需进一步研究。
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| 关 键 词： | 鸡 鹌鹑 杂交早期胚胎 性别鉴定 |
| 收稿时间： | 2006-11-09 |
| 修稿时间： | 2007-02-08 |
Identification of Sex of Earlier Embryos from Generic Hybrids of Chicken-Quail Using Gene Wpkci |
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| QIAO Ai-jun,MA Wen-xia,LI Da-quan,MENG Qing-mei.Identification of Sex of Earlier Embryos from Generic Hybrids of Chicken-Quail Using Gene Wpkci[J].Scientia Agricultura Sinica,2008,41(3):841-845. |
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| Authors: | QIAO Ai-jun MA Wen-xia LI Da-quan MENG Qing-mei |
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| Abstract: | Abstract]We had selected the earlier embryos of generic hybridization of chicken and quail (2.75-5.0 d),designed primers from quail Wpkci and β- actin genes,and used RT-PCR method to establish a way to identify the sex of earlier embryos of hybridization of the chicken(♂) and quail(♀).Amplified cDNA of quails of known sex and hybridizations’embryos of unknown sex with Wpkci primers ,we had acquired two particular segments of 402 bp and 296 bp from the female embryos, but none from the male embryos. In order to verify credibility and stability of this system deeply, we had acquired three particular segments of 490 bp,402bp,296bp from the female embryos with multi-ply PCR, but only acquired the internal standard segment of 490 bp from male embryos.Based on the optimization of the key factors of the experiment, we had established a simple,fast,credible and stable method to identify the sex of earlier hybridizations 'embryos. |
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| Keywords: | Chicken Quail Hybrid embryos Sexual identification |
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