The production of yellow-seeded Brassica napus (AACC) through crossing interspecific hybrids of B. campestris (AA) and B. carinata (BBCC) with B. napus |
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| Authors: | Jinling Meng Suwen Shi Li Gan Zaiyung Li Xinshun Qu |
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| Institution: | (1) National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China (author for correspondence);(2) Agronomy Department, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China;(3) Basic Courses Department, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China |
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| Abstract: | To transfer the genes for yellow seed coat from both genomes A and C to B. napus (AACC), the hexaploid of Brassica (AABBCC)
was synthesised from reciprocal interspecific crosses between yellow-seeded B.campestris (AA) and B.carinata (BBCC). The hexaploid
with 27 pairs of chromosomes was red-seeded which showed that genic interaction existed in the trigenomic plants for the colour
of the seed coat. Hundreds of hybrid seeds were obtained from crosses between the red-seeded hexaploid and partial yellow
or brown-seeded varieties of B. napus as pollen donor. The majority of the hybrid plants (AABCC) were self fertile with brown
seeds. It appeared that the chromosomes of the B genome were excluded during the meiosis of the pentaploid and a high proportion
of the genetically balanced AC gametes could be produced. The fertility of the F2 population was increased and even reached
normal levels for some plants. Seventy-three plants with the yellow-seeded character were isolated from 2590 open-pollinated
F2 plants, most with increased fertility. After two successive self-pollinations, 18 lines produced yellow seeds and no brown
seeds segregated from these populations. The morphology of the novel yellow-seeded plants was basically towards B. napus.
Esterase isoenzyme electrophoresis showed that the plants contained some of the genetic background of B. campestris, B. carinata
and B. napus. Cytological analysis has shown that at least some yellow-seeded lines have the B.napus AACC genome composition
with 38 chromosomes and normal meiotic pairing.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | Brassica napus yellow-seed coat B campestris and B carinata interspecific hybridization hexaploid (AABBCC) pentaploid (AABCC) |
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