| 层出镰孢菌GD-5固态发酵培养条件及对藜和密花香薷的除草活性 |
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| 引用本文： | 朱海霞,马永强,郭青云.层出镰孢菌GD-5固态发酵培养条件及对藜和密花香薷的除草活性[J].植物保护学报,2018,45(5):1154-1160. |
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| 作者姓名： | 朱海霞 马永强 郭青云 |
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| 作者单位： | 青海大学农林科学院, 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 农业部西宁作物有害生物科学观测实验站, 青海省农业有害生物综合治理重点实验室, 西宁 810016,青海大学农林科学院, 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 农业部西宁作物有害生物科学观测实验站, 青海省农业有害生物综合治理重点实验室, 西宁 810016,青海大学农林科学院, 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 农业部西宁作物有害生物科学观测实验站, 青海省农业有害生物综合治理重点实验室, 西宁 810016 |
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| 基金项目： | 国家自然科学基金（31560518），中国科学院“西部之光”人才培养引进计划（201523） |
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| 摘 要： | 为获得层出镰孢菌Fusarium proliferatum菌株GD-5的最适固态发酵培养条件,采用单因素法筛选该菌株最适生长的碳氮源、固体发酵基质,以L_(25)(5~7)正交设计法确定最佳培养条件,并通过离体叶片法、盆栽试验和田间小区试验测定其对藜和密花香薷2种杂草的除草活性。结果表明,该菌株最适生长的固体发酵基质为麦麸,最适碳氮源分别是玉米粉和(NH_4)_2SO_4。优化后的固态培养条件为:N源(NH4)_2SO_443.0 mg/g、C源玉米粉48.4 mg/g、培养基质初始含水量258 mg/g、接种量0.26 mL/g、初始p H 7.6、培养时间120 h、培养温度30.7℃。优化得到的菌株在藜和密花香薷离体叶片、苗期盆栽和田间小区接种试验中均表现出较强的致病性,在盆栽试验中菌株GD-5对藜的致病率和鲜重防效分别为88.20%和69.46%,高于对密花香薷的79.70%和60.84%;在小区试验中对密花香薷的致病率和鲜重防效分别是91.40%和90.69%,高于对藜的64.50%和50.28%。说明该菌株具有进一步开发成为生物除草剂的潜力,有望替代或部分替代化学除草剂,防治农田杂草藜和密花香薷。
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| 关 键 词： | 层出镰孢GD-5 正交设计 培养条件 除草活性 |
| 收稿时间： | 2018/4/11 0:00:00 |
Solid culture conditions of Fusarium proliferatum GD-5 and its herbicidal activity to weeds Chenopodium album and Elsholtzia densa |
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| Zhu Haixi,Ma Yongqiang and Guo Qingyun.Solid culture conditions of Fusarium proliferatum GD-5 and its herbicidal activity to weeds Chenopodium album and Elsholtzia densa[J].Acta Phytophylacica Sinica,2018,45(5):1154-1160. |
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| Authors: | Zhu Haixi Ma Yongqiang and Guo Qingyun |
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| Institution: | Key Laboratory of Agricultural Integrated Pest Management of Qinghai Province, Scientific Observation and Experimental Station of Crop Pests in Xining, Ministry of Agriculture;State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, Qinghai Province, China,Key Laboratory of Agricultural Integrated Pest Management of Qinghai Province, Scientific Observation and Experimental Station of Crop Pests in Xining, Ministry of Agriculture;State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, Qinghai Province, China and Key Laboratory of Agricultural Integrated Pest Management of Qinghai Province, Scientific Observation and Experimental Station of Crop Pests in Xining, Ministry of Agriculture;State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, Qinghai Province, China |
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| Abstract: | |
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| Keywords: | Fusarium proliferatum GD-5 orthogonal design culture condition herbicidal activity |
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