| 急性氨氮毒性对黄颡鱼头肾巨噬细胞抗氧化及炎症相关基因表达的影响 |
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| 引用本文： | 李冰,张木子,黎明,袁莉霞,王日昕.急性氨氮毒性对黄颡鱼头肾巨噬细胞抗氧化及炎症相关基因表达的影响[J].水产学报,2018,42(12):1889-1895. |
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| 作者姓名： | 李冰 张木子 黎明 袁莉霞 王日昕 |
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| 作者单位： | 宁波大学海洋学院, 浙江 宁波 315211,宁波大学海洋学院, 浙江 宁波 315211,宁波大学海洋学院, 浙江 宁波 315211,宁波大学海洋学院, 浙江 宁波 315211,宁波大学海洋学院, 浙江 宁波 315211 |
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| 基金项目： | 国家自然科学基金（31502176，31472279）；宁波市自然科学基金（2016A610083） |
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| 摘 要： | 为了研究急性氨氮胁迫对黄颡鱼头肾巨噬细胞抗氧化及炎症相关基因表达的影响，实验通过建立黄颡鱼离体头肾细胞模型，开展为期96 h的急性氨氮胁迫实验。结果显示，0.14和0.28 mg/L总氨氮处理组SOD基因的相对表达量显著低于对照组，但0.28 mg/L总氨氮处理组GPX基因的相对表达量显著高于其他组；0.14和0.28 mg/L总氨氮处理组IL-1和TNF基因的相对表达量显著低于对照组，而0.28 mg/L总氨氮处理组IL-8基因的相对表达量显著低于0.14 mg/L总氨氮处理组和对照组；吖啶橙染色检测发现，0.14 mg/L总氨氮处理组实验鱼头肾巨噬细胞内可见边缘不规则的黄绿色荧光，0.28 mg/L总氨氮处理组实验鱼头肾巨噬细胞内可见致密浓染的黄绿色荧光信号。研究表明，氨氮毒性能够影响黄颡鱼头肾巨噬细胞抗氧化相关基因的表达；细胞凋亡可能是造成鱼类氨中毒致死的主要原因之一。
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| 关 键 词： | 黄颡鱼 头肾巨噬细胞 氨氮 抗氧化 炎症 细胞凋亡 |
| 收稿时间： | 2017/9/13 0:00:00 |
| 修稿时间： | 2018/1/15 0:00:00 |
Effect of acute ammonia toxicity on genes involved in antioxidant and inflammation in head kidney macrophage of Pelteobagrus fulvidraco |
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| LI Bing,ZHANG Muzi,LI Ming,YUAN Lixia and WANG Rixin.Effect of acute ammonia toxicity on genes involved in antioxidant and inflammation in head kidney macrophage of Pelteobagrus fulvidraco[J].Journal of Fisheries of China,2018,42(12):1889-1895. |
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| Authors: | LI Bing ZHANG Muzi LI Ming YUAN Lixia and WANG Rixin |
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| Institution: | School of Marine Sciences, Ningbo University, Ningbo 315211, China,School of Marine Sciences, Ningbo University, Ningbo 315211, China,School of Marine Sciences, Ningbo University, Ningbo 315211, China,School of Marine Sciences, Ningbo University, Ningbo 315211, China and School of Marine Sciences, Ningbo University, Ningbo 315211, China |
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| Abstract: | A study was carried out to test the response of genes involved in antioxidant and inflammation in head kidney macrophage to acute ammonia toxicity for 96 h. 0.14 and 0.28 mg/L total ammonia nitrogen (TA-N) groups had lower mRNA expression of SOD than that of fish in control, but the highest mRNA expression of GPX was found in 0.28 mg/L TA-N group; 0.14 and 0.28 mg/L TA-N groups had lower mRNA expression of IL-1 and TNF than those of control group, and 0.28 mg/L TA-N group had lower mRNA expression of IL-8 than that of fish in 0.14 mg/L TA-N and control groups; detection with acridine orange staining showed that irregular acridine signal were detected in fish head kidney macrophage cells (HKMC) exposed to 0.14 mg/L TA-N, while HKMC treated with 0.28 mg/L TA-N exhibited extremely high fluoresence intensity. This study indicated that ammonia exerts its toxic effects by interfering with the mRNA expression of antioxidant genes; the apoptosis may be one of the main causes of death caused by ammonia poisoning. |
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| Keywords: | Pelteobagrus fulvidraco head-kidney macrophage ammonia antioxidant inflammation apoptosis |
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