Soybean Genotypic Variation for Nitrogen Fixation |
| |
Authors: | G B Reddy Alexandra Faza |
| |
Institution: | Department of Plant Science and Technology, North Carolina A &T State University, Greensboro, NC 27411, U.S.A. |
| |
Abstract: | Eighteen soybean ( Glycine max L.] Merr) cultivars from maturity groups V and VI were screened with two Bradyrhizobium japonicum strains (USDA 110 and TAL 378) in the greenhouse to determine N2 fixation (C2H2: reduction) rates. Antibiotic resistant markers were used for observing nodule occupancy. Cultivars L.-76-0132, PI 159319, Hoberland, Lee, PI 22173 and Centennial showed high nitrogenase activity of 6.25, 5.54, 17.43, 4.73, 6.94, and 4.81 μM C2H4/Pl/hr, respectively. Cultivars PI417419, PI 230978, Ogden , and FC 31665 showed low nitrogenase activity of 1.51, 0.80, 0.62 and 0.94 μM C2H4/Pl/hr, respectively. The data from this screening experiment suggest that the N2 fixation varied among genotypes and all the nodules were occupied by the inoculated strains only. Rhizobial strain effect was observed among soybean cultivars (MGVI). |
| |
Keywords: | Glycine max acetylene reduction Bradyrhizobium japonicum nitrogen fixation nitrogenase activity |
|
|