Genetic Diversity Among Brazilian Cultivars and Landraces of Tomato Lycopersicon Esculentum Mill. Revealed by RAPD Markers |
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| Authors: | Bernardette Primieri Carelli Lee Tseng Sheng Gerald Felipe Gobbi Grazziotin Sergio Echeverrigaray |
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| Institution: | 1. Instituto de Biotecnologia, Universidade de Caxias do Sul, R. Francisco Getúlio Vargas, 1130, Caxias do Sul, 95001-970, RS, Brasil
2. Centro de Ciências Agrárias, Universidade Federal de S?o Carlos, Via Anhanguera, Km 174, Caixa Postal 153, Araras, SP, Brasil
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| Abstract: | Random amplified polymorphic DNA markers (RAPD) were used to estimate the variability of 35 tomato accessions (Lycopersicon esculentum Mill.). A total of 257 reproducibly scorable bands were obtained from 20 primers, 78.6% of which were polymorphic. The percentage
distribution of RAPD markers shows a bimodal distribution, and the frequency of rare alleles is similar in commercial and
landrace accessions. Genetic distances among accessions were calculated and a dendrogram showing the genetic relationships
among them was constructed allowing for the separation of four groups. Twenty out of 23 Brazilian landraces fell within one
group, whereas commercial cultivars were distributed in the four groups. AMOVA analysis of RAPD data showed that, despite
the high within Brazilian landraces and commercial cultivars variation, these two groups are significantly different, indicating
that landraces can be a source of variation for breeding programs. |
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| Keywords: | Genetic variability Lycopersicon esculentum Molecular markers |
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