| 一种新的非酶法分离荠幼胚的方法(英文) |
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| 引用本文： | 洪亚平,张亚冰,吴国锋,赵文娟,阎婵婵.一种新的非酶法分离荠幼胚的方法(英文)[J].Agricultural Science&Technology,2009(3). |
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| 作者姓名： | 洪亚平 张亚冰 吴国锋 赵文娟 阎婵婵 |
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| 作者单位： | 河南科技大学农学院; |
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| 基金项目： | Supported by Natural Science Foundation (No. 30770124);;SRTP Fund from Henan University of Science and Technology~~ |
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| 摘 要： | Objective] The aim of this study was to manually isolate the microstructures such as ovule and embryo of Capsella bursa-pastoris (L.) Medic. by the non-enzymatic method. Method] With the gum block and dissecting microscope, its floral characteristics, ovule and embryo were manually isolated and observed by the non-enzymatic method. Result] C. bursa-pastoris had four calyces, four petals, tetradynamous stamen and silicle, while its embryogenesis contained globular embryo, heart-shaped embryo and torpedo-shaped embryo. Conclusion] The microstructures such as ovule and embryo of plants become easier to be isolated manually with the viscosity of gum block, which has small damage on embryo or disadvantages of keeping original shapes, and also can used in multi fields studies. This test can also be accomplished in labs with poor experiment facilities.
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| 关 键 词： | 幼胚 分离 蒸馏水 胶块 自来水 非酶法 种子 形态观察 解剖针 子房室 |
A New Non-enzymatic Method to Isolate Immature Embryos of Capsella bursa-pastoris (L.) Medic. |
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| HONG Ya-ping,ZHANG Ya-bing,WU Guo-feng,ZHAO Wen-juan,YAN Chan-chan College of Agronomy,Henan University of Science , Technology,Luoyang.A New Non-enzymatic Method to Isolate Immature Embryos of Capsella bursa-pastoris (L.) Medic.[J].农业科学与技术(英文版),2009(3). |
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| Authors: | HONG Ya-ping ZHANG Ya-bing WU Guo-feng ZHAO Wen-juan YAN Chan-chan College of Agronomy Henan University of Science Technology Luoyang |
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| Institution: | HONG Ya-ping,ZHANG Ya-bing,WU Guo-feng,ZHAO Wen-juan,YAN Chan-chan College of Agronomy,Henan University of Science , Technology,Luoyang 471003 |
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| Abstract: | Objective] The aim of this study was to manually isolate the microstructures such as ovule and embryo of Capsella bursa-pastoris (L.) Medic. by the non-enzymatic method. Method] With the gum block and dissecting microscope, its floral characteristics, ovule and embryo were manually isolated and observed by the non-enzymatic method. Result] C. bursa-pastoris had four calyces, four petals, tetradynamous stamen and silicle, while its embryogenesis contained globular embryo, heart-shaped embryo and torpedo-s... |
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| Keywords: | Capsella bursa-pastoris (L ) Medic Ovule Embryo Gum block Isolation |
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