| 埃氏巨型球菌及其乙酸生成关键酶基因缺失工程菌体外代谢特性的初步研究 |
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| 引用本文： | 孙国权,刘国文,邢欣,逄晓阳,龙淼,苑学,杨文艳,王哲.埃氏巨型球菌及其乙酸生成关键酶基因缺失工程菌体外代谢特性的初步研究[J].中国畜牧兽医,2010,37(4):26-29. |
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| 作者姓名： | 孙国权 刘国文 邢欣 逄晓阳 龙淼 苑学 杨文艳 王哲 |
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| 作者单位： | 1.吉林大学畜牧兽医学院，长春 130062；2.内蒙古民族大学动物科技学院，通辽 028042；3.吉林农业大学动物科技学院，长春 130118；4.吉林省产品质量监督检验院，长春 130022） |
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| 基金项目： | 国家自然科学基金资助项目(30600441,30230260) |
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| 摘 要： | 埃氏巨型球菌是奶牛瘤胃中的一种优势菌。本研究运用瘤胃微生态理论,结合埃氏巨型球菌能利用乳酸发酵产生丙酸的特点,初步研究埃氏巨型球菌(H6)及其乙酸生成关键酶基因缺失工程菌(TnH6)体外发酵特性。经健康羊瘤胃液体外连续培养结果表明,埃氏巨型球菌及其乙酸生成关键酶基因缺失工程菌均能利用乳酸生成VFA,发酵生成的VFA以丙酸为主,发酵类型倾向于丙酸型,但工程菌生成乙酸的能力明显降低,尽管丙酸生成能力有所下降,但显著降低了乙酸与丙酸的比例,从而为调控瘤胃乳酸发酵和利用H6、TnH6防治奶牛亚临床性瘤胃乳酸酸中毒提供了理论和试验基础。
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| 关 键 词： | 埃氏巨型球菌 乳酸发酵 代谢特性 |
Metabolic Characterization of Megasphaera Elsdenii and its Gene Deletion Engineering Bacteria in vitro |
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| SUN Guo-quan,LIU Guo-wen,XING Xin,PANG Xiao-yang,LONG Miao,YUAN Xue,YANG Wen-yan,WANG Zhe.Metabolic Characterization of Megasphaera Elsdenii and its Gene Deletion Engineering Bacteria in vitro[J].China Animal Husbandry & Veterinary Medicine,2010,37(4):26-29. |
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| Authors: | SUN Guo-quan LIU Guo-wen XING Xin PANG Xiao-yang LONG Miao YUAN Xue YANG Wen-yan WANG Zhe |
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| Institution: | （1.College of Animal Science and Veterinary Medicine, Jilin University,Changchun 130062,China；2.College of Animal Science and Technology, Inner Mongolia National University,Tongliao 028042,China; 3.College of Animal Science and Technology, Jilin Agricultural University,Changchun 130118,China；4.Product Quality Supervision and Inspection Institute of Jilin Province, Changchun 130022,China） |
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| Abstract: | Megasphaera elsdenii is one of dominant bacteria in the rumen.In according to the characteristic of energy metabolism for the ruminant,that is energy supply through the volatile fatty acids such as acetate,propionate,butyrate generated by fermentation of microorganism in rumen,and the theory of ruminal fermentated regulation,which is mainly to regulate the degradation of lactate and the production of propionate.Primary substrates(lactic acid and pyruvate) were cocultured with H6 and TnH6 for 48 h,respective... |
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| Keywords: | Megasphaera elsdenii lactic fermentation metabolic characteristics |
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