Neuropeptide Y (NPY) modulatesin vitro gonadotropin in release from rainbow trout pituitary glands |
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| Authors: | Bernard Breton Tomasz Mikolajczyk Jean-Michel Danger Francis Gonnet Serge Saint-Pierre Hubert Vaudry |
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| Institution: | (1) Laboratoire de Physiologie des Poissons, INRA, Campus de Beaulieu, 35042 Rennes Cedex, France;(2) Groupe de Recherche en Endocrinologie Moléculaire, UA CNRS 650, Unité Affiliée à l'INSERM, Université de Rouen, 76134 Mont-Saint-Aignan, France;(3) Department of Ichthyobiology and Fisheries, Academy of Agriculture, Krakow-Midlinickik, Poland;(4) Institut National de la Recherche Scientifique, Departement Santé, Université du Québec, Pointe Claire, P.Q., H9R 1G6, Canada |
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| Abstract: | This work investigated the action of neuropeptide Y (NPY) on thein vitro pituitary release of the maturing gonadotropic hormone (GtH) of the rainbow trout using a perifusion system employing trout
balanced salt solution (pH 7.5) at 15°C and a 12.5 ml/h flow rate. In vitellogenic females a 20 minutes NPY application (10−7 M) induced a 20–30% decrease in GtH secretion. Removal of NPY was followed by a rebound in GTH secretion. On the contrary,
in ovulated females, NPY (15 minutes, 10−7 M) directly stimulated GTH secretion. The greatest stimulation was obtained the day of ovulation where the stimulatory effect
of NPY was similar to those induced by s.GnRH in the same conditions, reaching 400% of the basal GTH level. In vitellogenic
females treated with 1-4-6 androstadien 3–7 dione, an inhibitor of aromatase activity, the pituitary response to NPY was similar
to that obtained in ovulated females. Thus thein vitro action of NPY might depend on thein vivo steroidogenic environment. |
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| Keywords: | gonadotropin neuropeptide Y GnRH in vitro female trout |
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