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| 黑老虎枝枯病病原鉴定及其生物学特性 | |
| 引用本文： | 石春发,陶光林,胡岑龙,杨东红,郝磊,张喜.黑老虎枝枯病病原鉴定及其生物学特性[J].植物病理学报,2019,49(6):866-870. |
| 作者姓名： | 石春发 陶光林 胡岑龙 杨东红 郝磊 张喜 |
| 作者单位： | 黔东南州林业科学研究所,凯里556000; 贵州省林业科学研究院,贵阳550005 |
| 基金项目： | 贵州省林业厅林业优秀青年人才培养专项资金项目冷饭团病害调查及绿色防控技术研究(黔林科合J[2017]12号);国家重点研发计划项目喀斯特高原石漠化综合治理与生物能源产业技术与示范(2016YFC0502605);黔东南州科技局州级科技计划项目贵州省南五味子属种质资源收集(黔东南科合J字〔2018〕022号) |
| 摘 要： | <正>黑老虎Kadsura coccinea (Lem.) A. C. Smith]又名冷饭团、布福娜,果实含丰富的Vc、Ve及多种微量元素,是山区野果珍品1]。上世纪末,人们从野外引种试栽,目前在黔东南地区实现了规模化栽培。笔者于2017和2018年调查发现,黑老虎枝枯病在黔东南州各种植基地普遍发生,造成严重损失,但关于黑老虎病害的报道甚少2,3]。本研究对黑老虎枝枯病的病原菌进行了分离培养、柯赫氏法则验证、形态学特征观察及多基因序列 |
| 收稿时间： | 2018-12-11 |
Identification and characterization of causal organism of Kadsura coccinea twig blight |
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| SHI Chun-fa,TAO Guang-lin,HU cen-long,YANG Dong-hong,HAO Lei,ZHANG Xi.Identification and characterization of causal organism of Kadsura coccinea twig blight[J].Acta Phytopathologica Sinica,2019,49(6):866-870. | |
| Authors: | SHI Chun-fa TAO Guang-lin HU cen-long YANG Dong-hong HAO Lei ZHANG Xi |
| Institution: | Qiandongnan State Forestry Research Institute, Kaili 556000, China; Guizhou Academy of Forestry, Guiyang 550005, China |
| Abstract: | Kadsura coccinea (Lem.) A. C. Smith has been wildly cultivated in Southeast of Guizhou province, bearing fruit as an excellent nutrition source being rich in vitamin C, vitamin E, and a variety of trace elements. In 2017-2018, we found twig blight on Kadsura coccinea with small black dot-like structure in the late stage on lesion, a very common disease causing significant economic losses. The fungal isolates were recovered from the symptomatic stem tissues and small black dot (pycnidium) which contained transparent, unicellular and spindle-shaped conidia of (17.4±1.2) μm×(6.5±0.7) μm. The purified culture with the grayish white to dark grayish-green as well as partial straight hyphae on PDA plate was consistent with Neofusicoccum parvum, supported by the aliment and phylogenetic analyses of ITS, Ef1 α and Tub2 gene sequences (GenBank no: MK563984、MK563986 and MK563987).The pathogenicity test on leaves and stems with needle-punching method matched the symptoms described above and re-isolated the fungus confirming the Koch’s postulate. The lethal temperature for mycelial growth was 54℃ for 10 min; The strain could grow normally in most carbon and nitrogen sources except ammonium carbonate. This is the first report of Neofusicoccum parvum causing twig blight on Kadsura coccinea in China. |
| Keywords: | Kadsura coccinea twig blight Neofusicoccum parvum pathogenic fungus |
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