| 多浆旱生植物霸王高频组培再生体系的建立 |
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| 引用本文： | 冯波,段娇娇,伍国强,王锁民.多浆旱生植物霸王高频组培再生体系的建立[J].草业学报,2010,19(6):140-146. |
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| 作者姓名： | 冯波 段娇娇 伍国强 王锁民 |
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| 作者单位： | 兰州大学草地农业科技学院,甘肃 兰州730020 |
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| 基金项目： | 863计划(2006AA10Z126); 国家自然科学基金(30770347,30671488)资助 |
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| 摘 要： | 本研究以多浆旱生植物霸王为材料,通过对不同激素组合和外植体(子叶、茎、茎节、下胚轴)的研究,建立了一个稳定、高效的霸王组培再生体系。结果表明,霸王子叶是诱导愈伤组织的最佳外植体,茎节是诱导不定芽的最适外植体;最佳愈伤组织诱导培养基是含有0.1mg/L6-BA和1.0mg/LNAA的MS,子叶在此培养基上愈伤组织诱导率为100%,诱导时间为20d,优于已报道的30d;霸王茎节不定芽诱导的最适培养基为附加1.5mg/L6-BA和0.5mg/LNAA的MS,诱导率高达82.5%,诱导时间为7周,优于已报道的10周;诱导生根的最佳培养基为含1.0mg/LNAA和0.5mg/LIBA的1/2MS,生根率高达86.7%,比已报道的73.3%的生根率提高了13.4%,且在此培养基上13d开始出现不定根,早于已报道的20d;本试验从诱导愈伤组织到再生苗成株需(84±4)d。
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| 关 键 词： | 霸王 外植体 激素组合 愈伤组织 植株再生 |
| 收稿时间： | 1900-01-01; |
Establishment of a high frequency regeneration system for the succulent xerophyte Zygophyllum xanthoxylum |
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| FENG Bo,DUAN Jiao-jiao,WU Guo-qiang,WANG Suo-min.Establishment of a high frequency regeneration system for the succulent xerophyte Zygophyllum xanthoxylum[J].Acta Prataculturae Sinica,2010,19(6):140-146. |
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| Authors: | FENG Bo DUAN Jiao-jiao WU Guo-qiang WANG Suo-min |
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| Institution: | College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China |
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| Abstract: | The effect of compounding proportions of different hormones on explants(cotyledon,stem,eustipes,and hypocotyl) were studied in tissue cultures of the succulent xerophyte Zygophyllum xanthoxylum,and a stable,high-efficiency plant regeneration system was established.Cotyledons were the best type of explant for callus induction and eustipes were the best for adventitious bud induction.The best callus inducing medium for Z.xanthoxylum was MS containing 0.1 mg/L 6-BA and 1.0 mg/L NAA,in which the callus inductio... |
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| Keywords: | Zygophyllum xanthoxylum explant hormones combination callus plant regeneration |
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