| 不同苦瓜品种果肉中酚类物质含量及抗氧化能力比较(英文) |
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| 引用本文： | 黄龙,邓媛元,张名位,张雁,魏振承,张瑞芬,唐小俊.不同苦瓜品种果肉中酚类物质含量及抗氧化能力比较(英文)[J].农业科学与技术,2012(6):1263-1269. |
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| 作者姓名： | 黄龙 邓媛元 张名位 张雁 魏振承 张瑞芬 唐小俊 |
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| 作者单位： | 广东省农业科学院农业生物技术研究所/农业部功能食品重点开放实验室 |
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| 基金项目： | Supported by Guangdong International Cooperation Program (2010B050600005);Guangdong Comprehensive Strategic Cooperation Project of CAS (2009B091300135);Guangdong Natural Science Foundation (10251064001000006)~~ |
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| 摘 要： | 目的]比较不同苦瓜品种果肉中游离态和结合态多酚含量及其抗氧化能力差异。方法]选用14个代表性的苦瓜品种,分析其果肉中的游离态和结合态总酚含量及其单体酚类的组成,采用铁离子还原能力(FRAP,Ferric reducing/antioxidant power)、DPPH (1,1-二苯基-2-苦基苯肼自由基,1,1-diphenyl-2-picrylhydrazy)和ABTS+·(2, 2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸, 2, 2’-azinobis-3-ethylbenzothiazoline-6-sulphonic acid di-ammonium salt)自由基法测定其抗氧化能力差异,同时分析其多酚含量和抗氧化活性的关系。结果]14 个不同苦瓜品种的游离态酚、结合态酚和总酚含量分别介于157.58～382.92、6.46～54和175.27～413.79 mg GAE/100 g DW,变异系数分别为23.50%、61.04%和21.58%,游离态酚含量占总酚含量的91.34%,结合态酚含量仅占总含量的8.66%;14 个品种的总黄酮含量介于8.97～18.22 mg CE/100 g DW,变异系数为22.80%;苦瓜中主要单体酚类物质香草醛酸、表儿茶素和芦丁含量亦存在显著的品种间差异,品种间的变幅分别为1.83～9.29、35.17～114.52 和0.91～4.53mg/100 g DW,变异系数分别为43.85%、26.97%、33.09%。FRAP抗氧化能力为 272.16～713.32 mg TE/100 g DW,变异系数为 27.67%;DPPH 清除能力的 IC50值为 11.43～34.14 mg GAE/100 g DW,变异系数为35.10%;ABTS+·清除能力的 IC50值为 21.57～119.71 mg GAE/100 g DW,变异系数为 63.75%。总酚含量与 FRAP 值呈极显著正相关(P<0.01),与DPPH和ABTS+·清除能力的IC50值分别呈极显著负相关(P<0.01)。结论]14个不同苦瓜品种的游离态酚、结合态酚和总酚含量分别介于 157.58～382.92、6.46～54 和175.27～413.79 mg GAE/100 g DW,变异系数分别为23.50%、61.04%和21.58%,游离态酚含量占总酚含量的 91.34%,结合态酚含量仅占总含量的 8.66%;14个品种的总黄酮含量介于8.97～18.22 mgCE/100 g DW,变异系数为22.80%;苦瓜中主要单体酚类物质香草醛酸、表儿茶素和芦丁含量亦存在显著的品种间差异,品种间的变幅分别为1.83～9.29、35.17～114.52和0.91～4.53 mg/100 g DW,变异系数分别为43.85%、26.97%、33.09%。FRAP抗氧化能力为272.16～713.32 mg TE/100g DW,变异系数为27.67%;DPPH清除能力的IC50值为11.43～34.14 mg GAE/100 g DW,变异系数为35.10%;ABTS+·清除能力的IC50值为21.57～119.71 mg GAE/100 g DW,变异系数为63.75%。总酚含量与FRAP值呈极显著正相关(P<0.01),与DPPH和ABTS+·清除能力的IC50值分别呈极显著负相关(P<0.01)
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| 关 键 词： | 苦瓜 多酚 抗氧化作用 |
Comparisons between Phenolic Compounds and Antioxidation of Momordica charantia L. in Different Varieties |
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| Long HUANG , Yuanyuan DENG, Mingwei ZHANG, Yan ZHANG, Zhencheng WEI, Ruifen ZHANG, Xiaojun TANG.Comparisons between Phenolic Compounds and Antioxidation of Momordica charantia L. in Different Varieties[J].Agricultural Science ＆ Technology,2012(6):1263-1269. |
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| Authors: | Long HUANG Yuanyuan DENG Mingwei ZHANG Yan ZHANG Zhencheng WEI Ruifen ZHANG Xiaojun TANG |
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| Institution: | Key Laboratory of Functional Food of Ministry of Agriculture of Bio-technical Research Institute in Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China |
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| Abstract: | Objective] The aim was to compare contents of free and bound polyphenol and antioxidation of balsam pear in different varieties. Method] Fourteen varieties of balsam pear were chosen to analyze total contents of free or bound phenol and components of free phenol. FRAP (Freeic reducing/antioxidant power), DPPH (1,1diphenyl-2-picrylhydrazy), and ABTS +· (2, 2’-azinobis-3-ethylbenzothiazoline-6-sulphonic acid diammonium salt) were used to measure antioxidation and analyze relationship between polyphenol content and antioxidation. Result] Among the fourteen varieties of balsam pear, free, bound and total phenols were 157.58 -382.92, 6.46 -54 and 175.27 -413.79 mg GAE/100 g DW; coefficients of variance were 23.50% , 61.04% and 21.58%, respectively; free phenol accounted for 91.34% of total phenol and bound phenol accounted for 8.66% ; contents of total flavone for the fourteen varieties were from 8.97 to 18.22 mg CE/100 g DW and the coefficient was at 22.80%; vanillic aldehyde acid, epicatechin and rutin differed in contents among different varieties, which changed in 1.83-9.29, 35.17-114.52 and 0.91-4.53 mg/100 g DW and the coefficients were 43.85%, 26.97% and 33.09%; FRAP antioxidation was at 272.16 -713.32 mg TE/100 g DW and coefficient at 27.67% ; IC 50 of DPPH and ABTS +· clearance were 11.43-34.14 and 21.57-119.71 mg GAE/100 g DW and coefficients were at 35.10% and 63.75% , accordingly; content of total phenol was of extremely positive correlation with FRAP (P<0.01) and of extremely negative correlation with IC 50 of DPPH and ABTS + · clearance (P<0.01). Conclusion] Contents of phenolic compounds and antioxidation are of significant genotype differences; and phenolic substance and antioxidation are mainly in free form and phenolic substance is the major basis for antioxidation. |
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| Keywords: | Momordica charantia L Phenolic compounds Antioxidation |
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