| 产类细菌素乳酸菌的筛选及培养条件优化 |
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| 引用本文： | 陈宏伟,地力扎尔·阿扎提,张秀萍.产类细菌素乳酸菌的筛选及培养条件优化[J].塔里木农垦大学学报,2013(4):12-17. |
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| 作者姓名： | 陈宏伟 地力扎尔·阿扎提 张秀萍 |
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| 作者单位： | [1]新疆生产建设兵团畜牧科技重点实验室,阿拉尔新疆843300 [2]塔里木大学动物科学学院,阿拉尔新疆843300 |
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| 基金项目： | 新疆生产建设兵团畜牧科技重点实验室项目（HS201008）;塔里木大学大学生创新项目（TDZGCX11005）. |
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| 摘 要： | 从紫花苜蓿青贮、牛奶及奶酪、酸菜中的分离到4株可产生类细菌素菌株，即戊糖乳杆菌（Lactobacilluspentosus）、植物乳酸菌（Lactobacillusplantarum）、戊糖片球菌（Pediococcuspentosaceus）、丙酸杆菌（Propionibacterium）。粗提4株细菌的发酵液对金黄色葡萄球菌有较强的抑制生长作用，对大肠杆菌的抑制作用较弱。4株分离菌类细菌素在80℃热处理15min后活性基本不变，100oC处理15min仍保持一定活性；在pH3～5范围内抑菌活性较强。胃蛋白酶、胰蛋白酶均可使该类细菌素完全失活；酸性蛋白酶不能使其失活。对分离株戊糖乳杆菌培养条件优化后，在30℃（pH值为6．0）条件下，选择碳氮质量分数为5％、碳氮质量比为3：2，添加MgS040．07g／100ml，MnSO4 0．05g／100ml，0．2ml／100mlTween-80有利于类细菌素的产生，抑菌直径可达18．2mm，是传统培养基配方抑菌效果的1．2倍。
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| 关 键 词： | 乳酸菌 分离 类细菌素 |
Study on Screening of Bacteriocin -producing Lactobacillus and Optimization of Culture Conditions |
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| Cheng Hongwei,＇,Dilizhaer Azhati,Zhang Xiuping.Study on Screening of Bacteriocin -producing Lactobacillus and Optimization of Culture Conditions[J].Journal of Tarim University of Agricultural Reclamation,2013(4):12-17. |
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| Authors: | Cheng Hongwei ＇ Dilizhaer Azhati Zhang Xiuping |
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| Institution: | 1＇2. (1 Tarim Key Laboratory of Animal Husbandry Scienee and Technology, Xinjiang Crops of Produetion and Construction, Alar, Xinjiang 843300) (2 College of Animal Seience and Teehnology, Tarim University, Alar, Xinjiang 843300) |
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| Abstract: | Four Lactobacillus strains, which produce bacteriocin - like substance, were isolated from Medicago sativa silage, milk, cheese, and pickles. They are Lactobacillus pentosu, Lactobacillus plantarum, Pediococcus pentosaceus, Propionibacterium. Fermentation liquids exhibits strong inhibitory activity against staphylococcus au- reus, but less obvious to E. coli. Their Bacteriocins were resistant to temperature and pH values , but decreased to proteolytic enzymes. Culture medium was optimized and it was much more suitable for the growth. By incubating the Lactobacillus pentos can produce more bacteriocin - like substance in condition of 3：2 carbon - nitrogen mass ratio（C / N）, 0. 07 g/100ml MgSO4,0. 05 g/100ml MnSO4, 0. 2 ml/10Oml Tween-80. It was 1. 2 times than traditional formula could. |
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| Keywords: | Lactobacillus isolation bacteriocin ＇ like substance |
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