| 发酵香菇渣对断奶仔猪生长性能、结肠挥发性脂肪酸含量及微生物结构的影响 |
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| 引用本文： | 彭俏丽,柒启恩,周国勇,唐民,陈冬灵,刘燊,张辉华.发酵香菇渣对断奶仔猪生长性能、结肠挥发性脂肪酸含量及微生物结构的影响[J].中国畜牧兽医,2021,48(11):3985-3995. |
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| 作者姓名： | 彭俏丽 柒启恩 周国勇 唐民 陈冬灵 刘燊 张辉华 |
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| 作者单位： | 1. 佛山科学技术学院生命科学与工程学院, 佛山 528225;2. 越好生物科技(广州)股份有限公司, 广州 510700;3. 广东壹号食品股份有限公司, 湛江 524000 |
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| 基金项目： | 国家重点研发计划专项（2018YFD0501202-3）；广东省教育厅高校科研项目（2017GCZX006）；广东省科技计划项目（2015A020209） |
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| 摘 要： | 为研究发酵香菇渣（fermented shiitake residues，FSR）对断奶仔猪生产性能、十二指肠消化酶活性、空肠紧密连接蛋白相关基因表达、结肠挥发性脂肪酸及结肠微生物区系的影响，本研究选用100头健康的壹号土猪断奶仔猪（小耳花猪×杜洛克猪，公母各50头），随机分为2组，每组5个重复，每个重复10头猪，进行饲养试验。对照组饲喂基础饲粮（NC组），试验组饲喂基础饲粮+5%发酵香菇渣。试验期33 d。饲养试验结束时，从每个组中选择6头仔猪（公母各半）进行屠宰取样。检测指标包括生长性能、十二指肠黏膜消化酶活性、空肠紧密连接蛋白mRNA相对表达量、结肠挥发性脂肪酸含量和肠道微生物结构。结果显示，与对照组相比，试验组显著提高了断奶仔猪的末重和平均日增重（P<0.05），显著降低了料重比（P<0.05）；显著提高了十二指肠黏膜胰蛋白酶和β-淀粉酶活性（P<0.05）；显著提高了结肠挥发性脂肪酸丙酸、丁酸、异丁酸和异戊酸水平（P<0.05）；显著增加了结肠纤维杆菌门（Fibrobacteres）和链球菌属（Streptococcus）的相对丰度（P<0.01；P<0.05），对空肠紧密连接蛋白1（TJP1）和紧密连接蛋白2（TJP2）基因mRNA的表达无显著影响（P>0.05）。综上所述，饲粮中添加5%发酵香菇渣可提高断奶仔猪肠道消化酶活性，增加结肠挥发性脂肪酸含量，调节肠道微生物区系组成，有利于提高断奶仔猪的生长性能。
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| 关 键 词： | 断奶仔猪 发酵香菇渣 肠道健康 生长性能 肠道微生物结构 |
| 收稿时间： | 2021-05-24 |
Effects of Fermented Shiitake Residues on Growth Performance,Colonic Volatile Fatty Acids Contents and Microbial Structure in Weaned Piglet |
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| PENG Qiaoli,QI Qien,ZHOU Guoyong,TANG Min,CHEN Dongling,LIU Shen,ZHANG Huihua.Effects of Fermented Shiitake Residues on Growth Performance,Colonic Volatile Fatty Acids Contents and Microbial Structure in Weaned Piglet[J].China Animal Husbandry & Veterinary Medicine,2021,48(11):3985-3995. |
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| Authors: | PENG Qiaoli QI Qien ZHOU Guoyong TANG Min CHEN Dongling LIU Shen ZHANG Huihua |
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| Institution: | 1. School of Life Science and Engineering, Foshan University, Foshan 528225, China;2. Yuehao Biotechnology Co., Ltd., Guangzhou 510700, China;3. Guangdong No. 1 Food Co., Ltd., Zhanjiang 524000, China |
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| Abstract: | This study was carried out to investigate the effects of fermented shiitake residues (FSR) on growth performance, intestinal digestive enzyme activities, colonic volatile fatty acid contents and microbial structure in weaned piglets. A total of 100 weaned piglets (Small ear flower pigs×Duroc pigs, 50 males and 50 females) were allocated to two treatment groups with five replicates of ten pigs each. The piglets in control group were fed with the basal diet without FSR, while in experimental group were fed with the basal diet supplemented with 5% FSR. The experiment lasted for 33 days. At the end of the experiment, 6 piglets (half male and half female) were selected from each treatment and slaughtered for sampling. The growth performance, duodenal mucosal digestive enzyme activity, relative expression of jejunal tight junction protein (TJP) mRNA, colonic volatile fatty acid content and intestinal microbial structure in weaned piglets were detected. The results showed that compared with control group, in experiment group, the final weight and average daily gain (ADG) were significantly increased, and the F/G was significantly decreased (P<0.05), the activities of trypsin and β-amylase were significantly increased (P<0.05), the levels of propionic acid, butyric acid, isobutyric acid and isovaleric acid in colonic were significantly increased (P<0.05), the relative abundance of Fibrobacteres and Streptococcus in colonic were significantly increased (P<0.01;P<0.05). The expression of TJP1 and TJP2 genes between the two groups was not significant difference (P>0.05). In conclusion, dietary supplementation with 5% FSR enhanced growth performance, improved intestinal digestive enzyme activities and production of volatile fatty acids and favorably modulated intestinal microbiota composition in weaned piglets. |
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| Keywords: | weaned piglet fermented shiitake residue intestinal health growth performance intestinal microbiota structure |
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