| 4 种观赏百合的组培快繁技术研究 |
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| 引用本文： | 杨懋勋,单振菊,陈志云,陈考科
.中山市森林病虫害防治检疫站袁广东中山曰.嘉汉林业有限公司袁广东广州.4 种观赏百合的组培快繁技术研究[J].广东农业科学,2012,39(2):37-39. |
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| 作者姓名： | 杨懋勋 单振菊 陈志云 陈考科
.中山市森林病虫害防治检疫站袁广东中山曰.嘉汉林业有限公司袁广东广州 |
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| 作者单位： | 中山火炬职业技术学院生物医药系;南沙出入境检验检疫局;中山市森林病虫害防治检疫站;嘉汉林业有限公司 |
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| 基金项目： | 中山火炬职业技术学院校级项目(2009Y18) |
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| 摘 要： | 以观赏百合莫娜、黄天霸、巴巴拉、索邦等4个品种的鳞片为材料进行组培快繁技术研究。结果表明:用75%酒精消毒10 s,0.1%升汞消毒10 min,再用无菌水清洗5次的灭菌效果最好,污染率最低;MS+6-BA 0.5 mg/L+NAA 1.0 mg/L为最佳诱导培养基;MS+6-BA 1.0 mg/L+NAA 0.5 mg/L为最佳增殖培养基;MS+IBA 0.5 mg/L为最佳生根培养基;蛭石∶黄心土=1∶1为最佳移栽基质。整套组培快繁技术在莫娜、黄天霸、巴巴拉、索邦等4个观赏百合品种中均适用。
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| 关 键 词： | 观赏百合 组织培养 快速繁殖 |
Study on tissue culture and rapid propagation technique of four ornamental lily |
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| YANG Mao-xun,SHAN Zhen-ju,CHEN Zhi-yun,CHEN Kao-ke.Study on tissue culture and rapid propagation technique of four ornamental lily[J].Guangdong Agricultural Sciences,2012,39(2):37-39. |
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| Authors: | YANG Mao-xun SHAN Zhen-ju CHEN Zhi-yun CHEN Kao-ke |
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| Institution: | 1.Department of Biomedicine,Zhongshan Torch Polytechnic,Zhongshan 528436,China;2.Nansha Entry-Exit Inspection and Quarantine Bureau,Guangzhou 5114574,China;3.Forest Pest Management and Quarantine Station of Zhongshan City,Zhongshan 528403,China;4.Sino-Forest Corporation,Guangzhou 510610,China) |
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| Abstract: | Tissue culture and rapid propagation technique of four ornamental lily,Lilium Mona,Lilium Manissa,Lilium Barbaresco and Lilium Sorbonne were studied.The results showed that sterilized 10 s by 75% alcohol,treated 10 min by 0.1% mercuric chloride,and then cleaned 5 times by sterile water,formed the best technique of sterilization with the lowest contamination rate.The best induction medium was MS+6-BA 0.5 mg/L+NAA 1.0 mg/L.The best multiplication medium was MS+6-BA 1.0 mg/L+NAA 0.5 mg/L.The best rooting medium was MS+IBA 0.5 mg/L.The best transplantation medium was vermiculite∶yellow soil=1∶1.The whole set of tissue culture and rapid propagation technique was suitable for the four ornamental lily in this study. |
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| Keywords: | ornamental lily tissue culture rapidpropagation |
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