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利迪链霉菌A02诱导番茄抗灰霉病作用机制研究——对植株防御酶系的影响
引用本文:刘宇,刘建华,刘伟成,裘季燕,刘德文,段玉玺.利迪链霉菌A02诱导番茄抗灰霉病作用机制研究——对植株防御酶系的影响[J].华北农学报,2007,22(2):152-155.
作者姓名:刘宇  刘建华  刘伟成  裘季燕  刘德文  段玉玺
作者单位:1. 沈阳农业大学,植物保护学院,辽宁,沈阳,110161;北京市农林科学院,植保环保所,北京,100097
2. 北京市农林科学院,植保环保所,北京,100097
3. 沈阳农业大学,植物保护学院,辽宁,沈阳,110161
基金项目:北京市科技计划 , 国家自然科学基金 , 北京市自然科学基金
摘    要:利用利迪链霉菌生防菌株A02发酵液和番茄灰霉病菌对番茄植株进行诱导和接种处理,利用分光光度法测定处理前和处理后1~5 d番茄叶片组织的主要防御酶系——苯丙氨酸解氨酶、过氧化物酶、过氧化氢酶和多酚氧化酶的活性变化。试验结果显示,不接种灰霉病菌而单独诱导处理、接种灰霉病菌后诱导处理和诱导处理后接种灰霉病菌这3种处理都能提高番茄植株各防御酶系的活性,后者酶活性峰值最高,对番茄植株灰霉病的抑制作用也最明显,控制效果达94.92%。

关 键 词:利迪链霉菌A02  诱导抗病性  番茄灰霉病  防御酶系
文章编号:1000-7091(2007)02-0152-04
修稿时间:2006-11-23

The Inducing Effect of Streptomyces lydicus A02 on Tomato Resistance to Botrytis cinerea-Impact on Defense Enzymes of Tomato Seedlings
LIU Yu,LIU Jian-hua,LIU Wei-cheng,QIU Ji-yan,LIU De-wen,DUAN Yu-xi.The Inducing Effect of Streptomyces lydicus A02 on Tomato Resistance to Botrytis cinerea-Impact on Defense Enzymes of Tomato Seedlings[J].Acta Agriculturae Boreali-Sinica,2007,22(2):152-155.
Authors:LIU Yu  LIU Jian-hua  LIU Wei-cheng  QIU Ji-yan  LIU De-wen  DUAN Yu-xi
Institution:1. Plant Protection College, Shenyang Agricultural University, Shenyang 110161, China; 2. Institute of Plant and Environment Protection, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China
Abstract:The study on inducing resistant effect of Streptomyces lydicus A02 to tomato gray mold were carried out in greenhouse.Tomato seedlings were treated via three methods: only inducing with fermentation broth of Streptomyces lydicus A02,inducing after inoculating with spore suspension of Botrytis cinerea,inoculating after inducing.The activities of 4 defense enzymes in tomato leaves were assayed by ultraviolet-visible absorption spectroscopy.The results showed that all the three treatments could increase the activities of defense enzymes,of which the treatment of inoculating after inducing presented a better inducing effect on 4 defense enzymes and a better control effect on tomato gray mold than inducing after inoculating did.The control effect was up to 94.92%.
Keywords:Streptomyces lydicus A02  Induced resistance  Botrytis cinerea  Defense enzymes
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