| 地八角根际溶磷菌溶磷能力及菌株特性研究 |
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| 引用本文： | 陆瑞霞,王小利,李显刚,姚拓,赵相勇.地八角根际溶磷菌溶磷能力及菌株特性研究[J].中国草地学报,2012,34(4):101-108. |
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| 作者姓名： | 陆瑞霞 王小利 李显刚 姚拓 赵相勇 |
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| 作者单位： | 1. 贵州省草业研究所,贵州 贵阳,550006
2. 甘肃农业大学草业学院,甘肃 兰州,730070 |
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| 基金项目： | 贵州省农业攻关项目"贵州优良豆科牧草根际高效复合接种剂的研制与开发"(黔科合NY字[2009]3067):贵州省农业攻关项目"贵州野生地八角引种选育及应用推广研究" |
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| 摘 要： | 采用PKO无机培养基,从地八角根际分离具有溶磷能力的菌株,通过溶磷圈法筛选出7株解磷能力较强的菌株进行深入研究。利用钼蓝比色法对菌株在液体培养条件下的溶磷能力进行测定,结果表明:筛选的菌株分解磷酸钙的能力差异不大,溶磷量在123.37～135.23μg/mL之间,各菌株的溶磷量与分泌有机酸量、培养介质pH值之间均不存在显著相关性;各菌株均具有分泌IAA能力,大多数菌落呈淡黄色或乳白色、不规则、不透明、扁平、无色素;除1株菌表现为中性外,其他6株表现为产碱性;所有菌株在以甘露醇、蔗糖、1/2甘露醇+1/2蔗糖、葡萄糖、木糖、麦芽糖取代蛋白胨的碳源培养基上均生长良好。其中,DBR2菌株经16SrDNA序列分析,初步鉴定为肠杆菌。
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| 关 键 词： | 地八角 溶磷菌 分离鉴定 溶磷性能 |
Capability of Dissolving Phosphate and Characteristics of Phosphate-dissolving Bacteria in Rhizosphere of Astragalus bhotanensis in Guizhou |
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| LU Rui-xia
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WANG Xiao-li
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LI Xian-gang
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YAO Tuo
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ZHAO Xiang-yong.Capability of Dissolving Phosphate and Characteristics of Phosphate-dissolving Bacteria in Rhizosphere of Astragalus bhotanensis in Guizhou[J].Chinese Journal of Grassland,2012,34(4):101-108. |
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| Authors: | LU Rui-xia WANG Xiao-li LI Xian-gang YAO Tuo ZHAO Xiang-yong |
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| Institution: | 1(1.Guizhou Institute of Prataculture,Guiyang 550006,China;2.Pratacultural College, Gansu Agricultural University,Lanzhou 730070,China) |
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| Abstract: | By using PKO culture media,inorganic phosphate dissolving bacteria was isolated from rhizosphere of Astragalus bhotanensis.Some strains with higher capacity of dissolving phosphate were identified by means of phosphorus hole,Further study on phosphorus dissolving capability of the strains with the molybdenum blue spectrophotometer indicated that there were little differences in phosphate-dissolving microorganisms capability,phosphate dissolving capacity was 123.37~135.23μg/mL.There were no obvious relationship between the phosphate dissolving capacity and pH,organic acid.Most of the strains had the ability to produce IAA.Seven stains were selected for morphological characteristics study,and most bacterial colony was pale yellow or milk-white,irregular,opaque,flat,non-pigment,In addition to a bacteria showing neutral,the others produced alkaline;all strains grew well in carbon source medium of mannitol,sucrose,1/2 mannitol +1/2 sucrose,glucose,xylose,maltose replaced peptone,strain DBR2 was identified as Enterobacter by 16S rDNA sequence analysis. |
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| Keywords: | Astragalus bhotanensis Phosphate-dissolving bacteria Isolation and identification Capacity of dissolving phosphate |
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