| 光皮桦茎叶cDNA文库构建及部分EST序列SSR分析 |
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| 引用本文： | 张敏,黄华宏,林二培,周厚君,王亚辉,童再康.光皮桦茎叶cDNA文库构建及部分EST序列SSR分析[J].北京林业大学学报,2012,34(4):58-61. |
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| 作者姓名： | 张敏 黄华宏 林二培 周厚君 王亚辉 童再康 |
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| 作者单位： | 亚热带森林培育国家重点实验室培育基地,浙江农林大学林业与生物技术学院;亚热带森林培育国家重点实验室培育基地,浙江农林大学林业与生物技术学院;亚热带森林培育国家重点实验室培育基地,浙江农林大学林业与生物技术学院;亚热带森林培育国家重点实验室培育基地,浙江农林大学林业与生物技术学院;亚热带森林培育国家重点实验室培育基地,浙江农林大学林业与生物技术学院;亚热带森林培育国家重点实验室培育基地,浙江农林大学林业与生物技术学院 |
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| 基金项目： | 基金项目:浙江省重大科技专项重点项目，浙江省林业科技重点项目 |
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| 摘 要： | 以光皮桦茎叶组织为材料，构建了cDNA文库。初级文库滴度为1.5×106pfu/mL，重组率达97.3%，插入片段大小在0.5～3.0 kb之间，平均长度约为1.3 kb，表明所构建的文库质量较高，可用于后续基因克隆及基因表达谱的研究。利用微卫星查找软件对获得的224条EST序列进行微卫星位点搜寻及其丰度、分布比较，发现47条序列含微卫星位点60个，占全部EST序列的26.80%；在所有SSRs中二碱基重复最多，为42个，占总数的70.00%，含三、四碱基重复分别占总数的28.30%和1.70%。通过对光皮桦EST序列中微卫星位点信息的发掘分析，为有针对性地设计EST SSR引物、进行遗传多样性分析奠定了基础。
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| 关 键 词： | 光皮桦 cDNA文库 SSR分析 |
| 收稿时间： | 1900-01-01 |
Library construction of cDNA and SSR analysis of partial ESTs for stem and leaf of Betula luminifera. |
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| ZHANG Min, HUANG Hua-hong, LIN Er-pei, ZHOU Hou-jun, WANG Ya-hui, TONG Zai-kang..Library construction of cDNA and SSR analysis of partial ESTs for stem and leaf of Betula luminifera.[J].Journal of Beijing Forestry University,2012,34(4):58-61. |
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| Authors: | ZHANG Min HUANG Hua-hong LIN Er-pei ZHOU Hou-jun WANG Ya-hui TONG Zai-kang |
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| Institution: | Nurturing Station for State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Linan,311300, P.R.China. |
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| Abstract: | A cDNA library of stem and leaf from Betula luminifera was constructed.The primary titer of cDNA library was about 1.5×106 pfu/mL,its recombinant efficiency reached 97.3%,and the size of insert DNA fragments ranged from 0.5 to 3.0 kb,with an average of 1.3 kb.The results indicated that it was a higher-quality cDNA library,and could be used in gene cloning and gene expression profile analysis.Distribution and frequency of SSRs were analyzed in 224 non-redundant ESTs from B.luminifera cDNA library,using online searching software.The results showed that 60 SSRs distributed in 47 EST sequences,accounting for 26.80% of all ESTs.Dinucleotide would be the major repeat types,accounting for 70.00% of the total number of acquired SSRs.The tri-nucleotide and tetra-nucleotide repeats accounted for 28.30% and 1.70% respectively.This research might lay the foundation for designing the targeted EST-SSR primers and genetic diversity analysis by mining the information of EST-SSR loci in B.luminifera EST sequence data. |
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| Keywords: | Betula luminifera cDNA library SSR analysis |
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