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网箱养殖大黄鱼诺卡氏菌病的初步研究
引用本文:王国良.网箱养殖大黄鱼诺卡氏菌病的初步研究[J].水产学报,2006,30(1):103-107.
作者姓名:王国良
作者单位:宁波大学生命科学与生物工程学院,浙江,宁波,315211
基金项目:浙江省自然科学基金;浙江省教育厅资助项目
摘    要:首次报道了大黄鱼诺卡氏菌病的发生情况。病鱼以体表和心、脾、肾等内脏出现白色结节为主要症状,平均死亡率15%。对病鱼进行细菌分离培养和光镜、电镜检查,均发现长或短的丝状分枝状杆菌。用分离菌株作回归感染,证实为该大黄鱼结节病的病原菌。对病原菌的基本生物学特性进行了研究,确定病原为诺卡氏菌。

关 键 词:大黄鱼  诺卡氏菌病  诺卡氏菌  网箱养殖
文章编号:1000-0615(2006)01-0103-05
收稿时间:2004-12-14
修稿时间:2004-12-14

Preliminary study on nocardiosis in cage-reared large croaker,Pseudosciaena crocea (Richardson)
WANG Guo-liang.Preliminary study on nocardiosis in cage-reared large croaker,Pseudosciaena crocea (Richardson)[J].Journal of Fisheries of China,2006,30(1):103-107.
Authors:WANG Guo-liang
Institution:College of Life- science and Bio- technology, Ningbo University, Ningbo 315211, China
Abstract:An epizootic in seawater cage-reared large yellow croaker, Pseudesciaena crocea, in China from September to November 2003 was caused by a Nocardiasp. The cumulative mortality was 15% and diseased fish were 16 months old with total length from 20 to 30 cm. Multiple, white nodules, 0.1-0.3 cm in diameter, were scattered on the heart, spleen and kidney. The morphology of isolated bacteria from Lowenstein-Jensen medium (LJM) and tryptic soy agar (TSA) were Gram-positive, branching, bead-like or long, slender, filamentous rods. Experimental infection indicated that the isolated bacterium was pathogen responsible for the mortalities and the bacterium colonizes the fish via skin or gill, and intragastric exposure to the pathogen was less effective as a route of infection. Based on the symptom of diseased fish and the growth characteristics, morphological, physiological, and biological properties of the bacterium, the isolated were identified as NocardiaWTB1] sp.
Keywords:Pseudosciaena crocea  nocardiosis  Nocardia  cage-rear
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