| 定性PCR方法检测转基因玉米MON810转化事件的研究 |
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| 引用本文： | 易小平,贺萍萍,夏启玉,肖苏生,谢翔,杨小亮,李美英,郭安平.定性PCR方法检测转基因玉米MON810转化事件的研究[J].热带作物学报,2014,35(12):2384-2390. |
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| 作者姓名： | 易小平 贺萍萍 夏启玉 肖苏生 谢翔 杨小亮 李美英 郭安平 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所 农业部热带作物生物技术与遗传资源利用重点实验室 海南海口571101;农业部转基因植物及植物用微生物环境安全监督检验测试中心,海南海口571101 |
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| 基金项目： | 南繁区生物安全监测预警及控制关键技术研究与示范（No. 201403075）；海南南繁区Bt水稻基因飘移风险监测与控制（No. 2014ZX08012004-011）；海南省自然科学基金（No. 311082）。 |
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| 摘 要： | 转基因玉米MON810(Yield Gard R)是孟山都公司通过DNA重组技术和微注射轰击研发的一种具有对欧洲玉米螟(ECB;Ostrinia nublialis)有特殊抗性的转基因玉米品系,目前在世界各地已经得到广泛种植,为加强对该品系玉米的安全管理,本研究旨在建立MON810品系玉米的转化事件特异性定性PCR检测方法。根据MON810的插入序列信息,在3′端的侧翼序列处设计定性PCR检测的引物,检测MON810在其他几种常见转基因作物混合样品的特异性。结果表明,该方法具有很好的特异性。同时检测该引物系统的扩增灵敏度,结果表明,检测引物的灵敏度可达0.1%。建立的MON810特异定性PCR检测方法经全国7家实验室的验证,进一步证实该方法能够特异地检测出样品中的MON810转化事件,检测方法的灵敏度可达0.1%,且检测结果具有良好的可重复性和可重现性。MON810转化事件定性PCR检测方法的建立可满足于抗虫转基因玉米MON810及其衍生品种生物安全管理的需要。
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| 关 键 词： | 转基因玉米MON810 定性PCR 灵敏度 特异性 |
Event-specific Detection Method of Transgenic Maize MON810 by Using Qualitative Polymerase Chain Reaction(PCR) |
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| YI Xiaoping,HE Pingping,XIA Qiyu,XIAO Susheng,XIE Xiang,YANG Xiaoliang,LI Meiying and GUO Anping.Event-specific Detection Method of Transgenic Maize MON810 by Using Qualitative Polymerase Chain Reaction(PCR)[J].Chinese Journal of Tropical Crops,2014,35(12):2384-2390. |
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| Authors: | YI Xiaoping HE Pingping XIA Qiyu XIAO Susheng XIE Xiang YANG Xiaoliang LI Meiying and GUO Anping |
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| Institution: | 1 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences/ Key Laboratory
of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture 2 Environmental Safety Supervision and Inspection Center for Genetically Modified Plants and Microorganism used in Plants, Ministry of Agriculture;1 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences/ Key Laboratory
of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture 2 Environmental Safety Supervision and Inspection Center for Genetically Modified Plants and Microorganism used in Plants, Ministry of Agriculture;1 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences/ Key Laboratory
of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture 2 Environmental Safety Supervision and Inspection Center for Genetically Modified Plants and Microorganism used in Plants, Ministry of Agriculture;1 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences/ Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture 2 Environmental Safety Supervision and Inspection Center for Genetically Modified Plants and Microorganism used in Plants, Ministry of Agriculture;1 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences/ Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture 2 Environmental Safety Supervision and Inspection Center for Genetically Modified Plants and Microorganism used in Plants, Ministry of Agriculture;1 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences/ Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture 2 Environmental Safety Supervision and Inspection Center for Genetically Modified Plants and Microorganism used in Plants, Ministry of Agriculture;1 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences/ Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture 2 Environmental Safety Supervision and Inspection Center for Genetically Modified Plants and Microorganism used in Plants, Ministry of Agriculture;1 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences/ Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture 2 Environmental Safety Supervision and Inspection Center for Genetically Modified Plants and Microorganism used in Plants, Ministry of Agriculture |
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| Abstract: | |
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| Keywords: | Genetically modified maize(Zea mays)MON810(YieldGard (R)) Qualitative polymerase chain reaction (PCR) Detection sensitivity Specifically |
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