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| 大肠杆菌acrA基因的克隆、表达、抗体制备及不同耐药水平大肠杆菌AcrA表达水平的检测 | |
| 引用本文： | 张海旺,张丽云,刘旭东,邓旭明,胡仲明,曾凡勤.大肠杆菌acrA基因的克隆、表达、抗体制备及不同耐药水平大肠杆菌AcrA表达水平的检测[J].中国预防兽医学报,2006,28(3):279-284. |
| 作者姓名： | 张海旺 张丽云 刘旭东 邓旭明 胡仲明 曾凡勤 |
| 作者单位： | 1. 河北北方学院,河北,张家口,075131 2. 河北省兽医药品监察所,河北,石家庄,040051 3. 吉林大学,畜牧兽医学院,吉林,长春,130062 4. 军事医学科学院军事兽医研究所,吉林,长春,130062 |
| 摘 要： | 检测不同耐药水平大肠杆菌AcrA表达水平，探讨耐药水平与AcrA蛋白表达水平之间的关系。对acrA基因进行克隆、表达、制备抗AcrA抗体，Western blot方法比较同耐药水平大肠杆菌AcrA表达水平。结果表明，多重耐药株SEMR的AcrA表达明显高于单药耐药株SEICI和SEICH以及质控株ATCC25922。因此SEMR多重耐药性的产生与AcrA的高效表达直接有关。 |
| 关 键 词： | 大肠杆菌 多重耐药 |
| 文章编号： | 1008-0589(2006)03-0279-06 |
| 收稿时间： | 2004-12-30 |
| 修稿时间： | 2004年12月30 |
Cloning and expression of acrA gene and preparation antibodies and inspect the level of AcrA expression in different drug resistance level in Escherichia coli |
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| ZHANG Hai-wang,ZHANG Li-yun,LIU Xu-dong,DENG Xu-ming,HU Zhong-ming,ZENG Fan-qin.Cloning and expression of acrA gene and preparation antibodies and inspect the level of AcrA expression in different drug resistance level in Escherichia coli[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(3):279-284. | |
| Authors: | ZHANG Hai-wang ZHANG Li-yun LIU Xu-dong DENG Xu-ming HU Zhong-ming ZENG Fan-qin |
| Institution: | 1. Hebei Northern College,Zhangjiakou 075131, China; 2. The Animal Medicine Supervise Institute of Hebei Province, Shijiazhuang 040051, China; 3. College of Animal Husbandry and Veterinary Medicine, Jilin University Changchun 130062,China; 4. Military Veterinary Research institute, Changchun 130062, China |
| Abstract: | To inspect the level of AcrA expression in different drug resistance level in Escherichia coil,and discuss the relationship of resistance level with the expression level of AcrA protein. The acrA gene was cloned, AcrA protein was expression, and the anti- AcrA protein antibodies was prepared, at the same time , the expression level of AcrA protein in different drug resistance level in Escherichia coli was compared by Westem blot. The results showed that the expression level of AcrA protein in muti-drug resistance strain SEMR was higher than single-drug resistance strain SEICI and SEICH, control strain ATCC25922. There were relativity in the muti-drug resistance of SEMR with the higher expression level of AcrA. |
| Keywords: | AcrA |
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